Antibiotic resistance heterogeneity and LasR diversity within Pseudomonas aeruginosa populations from pneumonia in intensive care unit patients.

Autor: Crémet L; Laboratoire UPRES EA3826, IRS2 - Nantes Biotech, Université de Nantes, Nantes, France; Service de Bactériologie-Hygiène, Pôle de Biologie, CHU de Nantes, Nantes, France. Electronic address: lise.cremet@chu-nantes.fr., Leroy AG; Laboratoire UPRES EA3826, IRS2 - Nantes Biotech, Université de Nantes, Nantes, France; Service de Bactériologie-Hygiène, Pôle de Biologie, CHU de Nantes, Nantes, France., Muller D; Laboratoire UPRES EA3826, IRS2 - Nantes Biotech, Université de Nantes, Nantes, France; Service d'Anesthésie Réanimation Chirurgicale, Pôle d'Anesthésie-Réanimation, CHU de Nantes, Nantes, France., Delanou S; Laboratoire UPRES EA3826, IRS2 - Nantes Biotech, Université de Nantes, Nantes, France., Burghelea A; Laboratoire UPRES EA3826, IRS2 - Nantes Biotech, Université de Nantes, Nantes, France., Broquet A; Laboratoire UPRES EA3826, IRS2 - Nantes Biotech, Université de Nantes, Nantes, France., Roquilly A; Laboratoire UPRES EA3826, IRS2 - Nantes Biotech, Université de Nantes, Nantes, France; Service d'Anesthésie Réanimation Chirurgicale, Pôle d'Anesthésie-Réanimation, CHU de Nantes, Nantes, France., Caroff N; Laboratoire UPRES EA3826, IRS2 - Nantes Biotech, Université de Nantes, Nantes, France.
Jazyk: angličtina
Zdroj: International journal of antimicrobial agents [Int J Antimicrob Agents] 2021 Jun; Vol. 57 (6), pp. 106341. Date of Electronic Publication: 2021 Apr 20.
DOI: 10.1016/j.ijantimicag.2021.106341
Abstrakt: This study investigated within-host heterogeneity of 66 Pseudomonas aeruginosa populations from pneumonia in 51 critically ill ventilated patients by examining 30 colonies per bronchoalveolar lavage (BAL). Differences in antibiotic susceptibility and quorum-sensing (QS) phenotypes were observed between the members of 14 (21.2%) and 10 (15.2%) populations, respectively. A significant association was found between QS deficiency and ceftazidime resistance. QS deficiency was associated with various lasR modifications, and was observed in 25 of 51 (49.0%) patients, including seven patients who received ≤48 h of ventilation. This study confirms the need to examine diverse colonies when analysing BAL cultures, particularly in β-lactam-exposed patients, to avoid missing ceftazidime- or imipenem-resistant isolates.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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