Fetal Programming Effects of a Mild Food Restriction During Pregnancy in Mice: How Does It Compare to Intragestational Ghrelin Administration?

Autor: Torres PJ; Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, e Instituto de Investigaciones en Ciencias de la Salud (INICSA; CONICET-UNC), Santa Rosa 1085, X5000ESU, Córdoba, Argentina., Luque EM; Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, e Instituto de Investigaciones en Ciencias de la Salud (INICSA; CONICET-UNC), Santa Rosa 1085, X5000ESU, Córdoba, Argentina., Di Giorgio NP; Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental, (IBYME; CONICET), Vuelta de Obligado 2490, Buenos Aires, C1428ADN, Argentina., Ramírez ND; Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, e Instituto de Investigaciones en Ciencias de la Salud (INICSA; CONICET-UNC), Santa Rosa 1085, X5000ESU, Córdoba, Argentina., Ponzio MF; Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, e Instituto de Investigaciones en Ciencias de la Salud (INICSA; CONICET-UNC), Santa Rosa 1085, X5000ESU, Córdoba, Argentina., Cantarelli V; Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, e Instituto de Investigaciones en Ciencias de la Salud (INICSA; CONICET-UNC), Santa Rosa 1085, X5000ESU, Córdoba, Argentina., Carlini VP; Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, e Instituto de Investigaciones en Ciencias de la Salud (INICSA; CONICET-UNC), Santa Rosa 1085, X5000ESU, Córdoba, Argentina., Lux-Lantos V; Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental, (IBYME; CONICET), Vuelta de Obligado 2490, Buenos Aires, C1428ADN, Argentina., Martini AC; Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, e Instituto de Investigaciones en Ciencias de la Salud (INICSA; CONICET-UNC), Santa Rosa 1085, X5000ESU, Córdoba, Argentina. acmartini2000@fcm.unc.edu.ar.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2021 Dec; Vol. 28 (12), pp. 3547-3561. Date of Electronic Publication: 2021 Apr 15.
DOI: 10.1007/s43032-021-00574-7
Abstrakt: To explore in mice if a 15% food restriction protocol during pregnancy programs the offspring postnatal development, with emphasis on reproductive function, and to assess if ghrelin (Ghrl) administration to mouse dams exerts effects that mimic those obtained under mild caloric restriction. Mice were 15% food-restricted, injected with 4 nmol/animal/day of Ghrl, or injected with the vehicle (control) thorough pregnancy. After birth, the pups did not receive further treatment. Pups born from food-restricted dams (FR pups) were lighter than Ghrl pups at birth, but reached normal weight at adulthood. Ghrl pups were heavier at birth and gained more weight than control pups (C pups). This effect was not associated with plasma IGF-1. FR pups showed a delay in pinna detachment and eye opening, while an advance was observed in Ghrl pups. FR pups showed also impairment in the surface-righting reflex. In both female FR and Ghrl pups, there was an advance in vaginal opening and, in adulthood, FR pups showed a significant decrease in their own litter size and plasma progesterone, and an increase in embryo loss. A delay in testicular descent was evident in male Ghrl pups. Changes in puberty onset were not associated with differences in the expression of Kiss1 in hypothalamic nuclei. Finally, in adulthood, FR pups showed a significant decrease in sperm quality. In conclusion, a mild food restriction thorough gestation exerted programming effects on the offspring, affecting also their reproductive function in adulthood. These effects were not similar to those of intragestational Ghrl administration.
(© 2021. Society for Reproductive Investigation.)
Databáze: MEDLINE
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