Colorimetric RT-LAMP and LAMP-sequencing forDetecting SARS-CoV-2 RNA in Clinical Samples.

Autor:	Herbst K; Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany., Meurer M; Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany., Kirrmaier D; German Cancer Research Center (DKFZ), Heidelberg, Germany., Anders S; Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany., Knop M; Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany.; German Cancer Research Center (DKFZ), Heidelberg, Germany.; DKFZ-ZMBH Alliance, Heidelberg, Germany., Thi VLD; Schaller Research Group, Department of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany.
Jazyk:	angličtina
Zdroj:	Bio-protocol [Bio Protoc] 2021 Mar 20; Vol. 11 (6), pp. e3964. Date of Electronic Publication: 2021 Mar 20 (Print Publication: 2021).
DOI:	10.21769/BioProtoc.3964
Abstrakt:	During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect viral RNA by conventional qPCR, we used reverse transcription loop-mediated isothermal amplification (RT-LAMP). We previously established colorimetric RT-LAMP assays on both purified and unpurified SARS-CoV-2 clinical specimens and further developed a multiplexed sequencing protocol (LAMP-sequencing) to analyze the outcome of many RT-LAMP reactions at the same time (Dao Thi et al. , 2020). Extending on this work, we hereby provide step-by-step protocols for both RT-LAMP assays and read-outs. Competing Interests: Competing interestsThe authors declare no competing interests. (Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.)
Databáze:	MEDLINE
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