Global coordination of metabolic pathways in Escherichia coli by active and passive regulation.

Autor: Kochanowski K; Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.; Life Science Zurich PhD Program on Systems Biology, Zurich, Switzerland., Okano H; Department of Physics, University of California at San Diego, La Jolla, CA, USA., Patsalo V; Department of Integrative Structural and Computational Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA, USA., Williamson J; Department of Integrative Structural and Computational Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA, USA., Sauer U; Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland., Hwa T; Department of Physics, University of California at San Diego, La Jolla, CA, USA.; Institute for Theoretical Science, ETH Zurich, Zurich, Switzerland.
Jazyk: angličtina
Zdroj: Molecular systems biology [Mol Syst Biol] 2021 Apr; Vol. 17 (4), pp. e10064.
DOI: 10.15252/msb.202010064
Abstrakt: Microorganisms adjust metabolic activity to cope with diverse environments. While many studies have provided insights into how individual pathways are regulated, the mechanisms that give rise to coordinated metabolic responses are poorly understood. Here, we identify the regulatory mechanisms that coordinate catabolism and anabolism in Escherichia coli. Integrating protein, metabolite, and flux changes in genetically implemented catabolic or anabolic limitations, we show that combined global and local mechanisms coordinate the response to metabolic limitations. To allocate proteomic resources between catabolism and anabolism, E. coli uses a simple global gene regulatory program. Surprisingly, this program is largely implemented by a single transcription factor, Crp, which directly activates the expression of catabolic enzymes and indirectly reduces the expression of anabolic enzymes by passively sequestering cellular resources needed for their synthesis. However, metabolic fluxes are not controlled by this regulatory program alone; instead, fluxes are adjusted mostly through passive changes in the local metabolite concentrations. These mechanisms constitute a simple but effective global regulatory program that coarsely partitions resources between different parts of metabolism while ensuring robust coordination of individual metabolic reactions.
(© 2021 The Authors. Published under the terms of the CC BY 4.0 license.)
Databáze: MEDLINE
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