| Autor: |
Malone-Povolny MJ; Department of Chemistry, University of North Carolina at Chapel Hill, CB3290, Chapel Hill, North Carolina 27599, United States., Bradshaw TM; Department of Chemistry, University of North Carolina at Chapel Hill, CB3290, Chapel Hill, North Carolina 27599, United States., Merricks EP; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, CB3290, Chapel Hill, North Carolina 27599, United States., Long CT; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, CB3290, Chapel Hill, North Carolina 27599, United States., Nichols TC; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, CB3290, Chapel Hill, North Carolina 27599, United States., Schoenfisch MH; Department of Chemistry, University of North Carolina at Chapel Hill, CB3290, Chapel Hill, North Carolina 27599, United States. |
| Abstrakt: |
The tissue response to polyurethane (PU)-coated implants employing active and/or passive FBR mitigation techniques was evaluated over a 28 day study in a diabetic swine model. Active FBR mitigation was achieved through the sustained release of nitric oxide (NO) from a mesoporous silica nanoparticle-doped PU coating. Passive FBR mitigation was achieved through the application of a foam- or fiber-based topcoat. These topcoats were designed to possess topographical features known to promote tissue integration with foam-coated implants having pore sizes of approximately 50 μm and fiber-coated implants consisting of fiber diameters of less than 1 μm. Nitric oxide-release profiles were minimally impacted by the presence of either topcoat. Inflammatory cell density and collagen density at the implant-tissue interface were assessed at 7, 14, 21, and 28 days following implantation. Nitric oxide-releasing implants had significantly lower inflammatory cell density and collagen density than non-NO-releasing controls. The presence of a topcoat did not significantly impact inflammatory cell density, though top-coated textured implants resulted in significantly lower collagen density, irrespective of NO release. Overall, coatings that combine NO release with surface texture demonstrated the greatest potential for tissue-based biomedical device applications. |
