Hemolytic Tests Exploring Factor H Functional Activities.

Autor:	Chabannes M; INSERM, UMRS 1138, 'Inflammation, Complement and Cancer' Team, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, Paris, France., Togarsimalemath SK; INSERM, UMRS 1138, 'Inflammation, Complement and Cancer' Team, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, Paris, France., Dragon-Durey MA; INSERM, UMRS 1138, 'Inflammation, Complement and Cancer' Team, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, Paris, France. marie-agnes.durey@aphp.fr.; Service d'Immunologie Biologique, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France. marie-agnes.durey@aphp.fr.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2227, pp. 69-81.
DOI:	10.1007/978-1-0716-1016-9_7
Abstrakt:	Impairment of the complement regulatory protein Factor H (FH) is implicated in the physiopathological mechanisms of different diseases like atypical hemolytic and uremic syndrome and C3 glomerulopathies. It may be due to genetic abnormalities or acquired with the development of autoantibodies. FH has several ligands; therefore, the exploration of its functions requires to perform different tests. Among them, two hemolytic tests are very useful because they give specific and complementary information about FH functions. The first one is dedicated to explore the FH capacity to dissociate the alternative pathway C3 convertase, whereas the second one is designed to explore the capacity of FH to bind cell surfaces and to protect them from complement attack. This chapter describes the procedures to perform these two hemolytic tests, exploring in a complementary way the FH functionality.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu