| Autor: |
Xia CL; Department of Gynecology and Obstetrics, Key Laboratory of Maternal‑Fetal Medicine of Liaoning Province, Key Laboratory of Obstetrics and Gynecology of Higher Education of Liaoning Province, Liaoning Centre for Prenatal Diagnosis, Research Center of China Medical University Birth Cohort, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China., Xiao SQ; Department of Nursing, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China., Yang X; Department of Gynecology and Obstetrics, Key Laboratory of Maternal‑Fetal Medicine of Liaoning Province, Key Laboratory of Obstetrics and Gynecology of Higher Education of Liaoning Province, Liaoning Centre for Prenatal Diagnosis, Research Center of China Medical University Birth Cohort, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China., Liu CX; Department of Gynecology and Obstetrics, Key Laboratory of Maternal‑Fetal Medicine of Liaoning Province, Key Laboratory of Obstetrics and Gynecology of Higher Education of Liaoning Province, Liaoning Centre for Prenatal Diagnosis, Research Center of China Medical University Birth Cohort, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China., Qiu H; Dian Diagnostics Group Co., Ltd., Hangzhou, Zhejiang 310030, P.R. China., Jiang HK; Department of Pediatrics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China., Li-Ling J; Jinxin Research Institute of Reproductive Medicine and Genetics, Jinjiang Maternal and Children's Health Care Hospital, Chengdu, Sichuan 610041, P.R. China., Lyu Y; Department of Gynecology and Obstetrics, Key Laboratory of Maternal‑Fetal Medicine of Liaoning Province, Key Laboratory of Obstetrics and Gynecology of Higher Education of Liaoning Province, Liaoning Centre for Prenatal Diagnosis, Research Center of China Medical University Birth Cohort, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China. |
| Abstrakt: |
Short rib‑polydactyly syndrome type III (SRPS3) is a lethal perinatal skeletal disorder consisting of polydactyly and multi‑system organ abnormalities. To further assess the pathogenicity of two pairs of compound heterozygotes and to search for novel molecular etiology, X‑rays and hematoxylin and eosin staining were conducted in three cases: Two retrospective samples and a newly identified patient with SRPS3. In addition, next‑generation sequencing was used to evaluate a fetus with SRPS3. Typical radiological features of the three cases included a long, narrow thorax with short ribs, shortened long bones, spurs at the metaphysis of the long bones and congenital bowing of the femurs. The present study also observed atypical histopathological changes, together with the absence of proliferation and abundance of retaining cartilage in the primary spongiosum. In addition, two novel compound heterozygous variants were identified in the dynein cytoplasmic 2 heavy chain 1 ( DYNC2H1 ) gene of the fetus: NM_001080463.1, c.6591_6593delTGG (chr11:103055738‑103055740); NM_001080463.1, c.7883T>C (chr11:103070000). The findings of the present study provided further confirmation of the pathogenicity of two compound heterozygous variants in two retrospective samples and identified novel compound heterozygous variants. These findings may improve our knowledge of the histopathological and radiological changes in patients with SRPS3 and the relative effects of DYNC2H1 variants. The findings of the present study may facilitate the clinical and molecular diagnosis of SRPS3. |
