Photobiomodulation combined with adipose-derived stem cells encapsulated in methacrylated gelatin hydrogels enhances in vivo bone regeneration.

Autor: Calis M; Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Hacettepe University, Ankara, Turkey. mertcalis@gmail.com., Irmak G; Department of Bioengineering, Faculty of Engineering, Hacettepe University, Ankara, Turkey.; Department of Bioengineering, Faculty of Engineering and Nature Sciences, Malatya Turgut Ozal University, Malatya, Turkey., Demirtaş TT; Department of Bioengineering, Faculty of Engineering, Hacettepe University, Ankara, Turkey.; Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey., Kara M; Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Hacettepe University, Ankara, Turkey., Üstün GG; Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Hacettepe University, Ankara, Turkey., Gümüşderelioğlu M; Department of Bioengineering, Faculty of Engineering, Hacettepe University, Ankara, Turkey.; Department of Chemical Engineering, Faculty of Engineering, Hacettepe University, Ankara, Turkey., Türkkanı A; Department of Histology and Embryology, School of Medicine, TOBB University, Ankara, Turkey., Çakar AN; Department of Histology and Embryology, School of Medicine, TOBB University, Ankara, Turkey., Özgür F; Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2022 Feb; Vol. 37 (1), pp. 595-606. Date of Electronic Publication: 2021 Apr 11.
DOI: 10.1007/s10103-021-03308-y
Abstrakt: Reconstruction of bone defects is still a significant challenge. The aim of this study was to evaluate the effect of application of photobiomodulation (PBM) to enhance in vivo bone regeneration and osteogenic differentiation potential of adipose-derived stem cells (ADSCs) encapsulated in methacrylated gelatin (GEL-MA) hydrogels. Thirty-six Sprague-Dawley rats were randomly separated into 3 experimental groups (n = 12 each). The groups were control/blank defect (I), GEL-MA hydrogel (II), and ADSC-loaded GEL-MA (GEL-MA+ADSC) hydrogel (III). Biparietal critical sized bone defects (6 mm in size) are created in each animal. Half of the animals from each group (n = 6 each) were randomly selected for PBM application using polychromatic light in the near infrared region, 600-1200 nm. PBM was administered from 10 cm distance cranially in 48 h interval. The calvaria were harvested at the 20th week, and macroscopic, microtomographic, and histologic evaluation were performed for further analysis. Microtomographic evaluation demonstrated the highest result for mineralized matrix formation (MMF) in group III. PBM receiving samples of group III showed mean MMF of 79.93±3.41%, whereas the non-PBM receiving samples revealed mean MMF of 60.62±6.34 % (p=0.002). In terms of histologic evaluation of bone defect repair, the higher scores were obtained in the groups II and III when compared to the control group (2.0 for both PBM receiving and non-receiving specimens; p<0.001). ADSC-loaded microwave-induced GEL-MA hydrogels and periodic application of photobiomodulation with polychromatic light appear to have beneficial effect on bone regeneration and can stimulate ADSCs for osteogenic differentiation.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)
Databáze: MEDLINE
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