Meta-omics analysis indicates the saliva microbiome and its proteins associated with the prognosis of oral cancer patients.

Autor: Granato DC; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., Neves LX; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., Trino LD; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., Carnielli CM; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., Lopes AFB; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., Yokoo S; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., Pauletti BA; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., Domingues RR; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., Sá JO; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., Persinoti G; Laboratório Nacional de Biorrenováveis, LNBr, CNPEM, Campinas, São Paulo, Brazil., Paixão DAA; Laboratório Nacional de Biorrenováveis, LNBr, CNPEM, Campinas, São Paulo, Brazil., Rivera C; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil., de Sá Patroni FM; Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, Brazil., Tommazetto G; Department of Biological and Chemical Engineering (BCE), Aarhus University, 82000 Aarhus, Denmark., Santos-Silva AR; Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba Universidade Estadual de Campinas (UNICAMP), Piracicaba, SP, Brazil., Lopes MA; Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba Universidade Estadual de Campinas (UNICAMP), Piracicaba, SP, Brazil., de Castro G Jr; Oncologia Clínica, Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Brandão TB; Instituto do Câncer do Estado de São Paulo, Octavio Frias de Oliveira, São Paulo, Brazil., Prado-Ribeiro AC; Instituto do Câncer do Estado de São Paulo, Octavio Frias de Oliveira, São Paulo, Brazil., Squina FM; Universidade de Sorocaba, Departamento de Processos Tecnológicos e Ambientais, São Paulo, Brazil., Telles GP; Universidade de Campinas, Instituto de Computação, Campinas, São Paulo, Brazil., Paes Leme AF; Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, São Paulo, Brazil. Electronic address: adriana.paesleme@lnbio.cnpem.br.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. Proteins and proteomics [Biochim Biophys Acta Proteins Proteom] 2021 Aug; Vol. 1869 (8), pp. 140659. Date of Electronic Publication: 2021 Apr 09.
DOI: 10.1016/j.bbapap.2021.140659
Abstrakt: Saliva is a biofluid that maintains the health of oral tissues and the homeostasis of oral microbiota. Studies have demonstrated that Oral squamous cell carcinoma (OSCC) patients have different salivary microbiota than healthy individuals. However, the relationship between these microbial differences and clinicopathological outcomes is still far from conclusive. Herein, we investigate the capability of using metagenomic and metaproteomic saliva profiles to distinguish between Control (C), OSCC without active lesion (L0), and OSCC with active lesion (L1) patients. The results show that there are significantly distinct taxonomies and functional changes in L1 patients compared to C and L0 patients, suggesting compositional modulation of the oral microbiome, as the relative abundances of Centipeda, Veillonella, and Gemella suggested by metagenomics are correlated with tumor size, clinical stage, and active lesion. Metagenomics results also demonstrated that poor overall patient survival is associated with a higher relative abundance of Stenophotromonas, Staphylococcus, Centipeda, Selenomonas, Alloscordovia, and Acitenobacter. Finally, compositional and functional differences in the saliva content by metaproteomics analysis can distinguish healthy individuals from OSCC patients. In summary, our study suggests that oral microbiota and their protein abundance have potential diagnosis and prognosis value for oral cancer patients. Further studies are necessary to understand the role of uniquely detected metaproteins in the microbiota of healthy and OSCC patients as well as the crosstalk between saliva host proteins and the oral microbiome present in OSCC.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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