Prevalence, characterization and antibiotic resistance of Shiga toxigenic Escherichia coli serogroups isolated from fresh beef and locally processed ready-to-eat meat products in Lagos, Nigeria.

Autor: Fayemi OE; Department of Biological Sciences, College of Basic and Applied Science, Mountain Top University, Kilometre 12, Lagos-Ibadan Expressway, Prayer City, Ogun State, Nigeria. Electronic address: oefayemi@mtu.edu.ng., Akanni GB; Department of Biological Sciences, College of Basic and Applied Science, Mountain Top University, Kilometre 12, Lagos-Ibadan Expressway, Prayer City, Ogun State, Nigeria; Department of Food Science, University of Pretoria, Private Bag X 20, Hatfield, 0028 Pretoria, South Africa., Elegbeleye JA; Department of Food Science, University of Pretoria, Private Bag X 20, Hatfield, 0028 Pretoria, South Africa; Department of Microbiology, Faculty of Science, University of Lagos, Akoka, Lagos, Nigeria., Aboaba OO; Department of Microbiology, Faculty of Science, University of Lagos, Akoka, Lagos, Nigeria., Njage PM; Centre for Genomic Epidemiology, Technical University of Demark, Denmark.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2021 Jun 02; Vol. 347, pp. 109191. Date of Electronic Publication: 2021 Mar 31.
DOI: 10.1016/j.ijfoodmicro.2021.109191
Abstrakt: Fresh beef and meat products have been implicated in outbreaks of Shiga toxin-producing Escherichia coli (STEC) worldwide. This study investigated the prevalence of E. coli O157: H7 and non-O157 STEC serogroups in fresh beef in the open market and street vended meat products (n = 180) in Lagos metropolis, Nigeria. A combination of culture media and immunomagnetic separation followed by typing for associated virulence factors and serotypes was performed. Antimicrobial susceptibility testing was performed on the isolated STEC serotypes using the disk diffusion method. A total of 72 STEC serogroup isolates were detected from 61 out of 180 samples. The O157 STEC serotypes were detected in fresh beef, suya, minced meat and tsire with prevalence of 20.8% while non-O157 STEC serogroups were detected in all the samples. Molecular typing revealed 25% (n = 18) of the STEC serogroups showed presence of all the stx1, stx2, eaeA, fliCH7 and rfbEO157 virulence factors while 54.2% (n = 39) possessed a combination of two virulence genes. Multidrug resistance was discovered in 23.6% (n = 17) of the total STEC serogroups. Locally processed ready-to-eat meat products in Lagos metropolis, Nigeria harbour potentially pathogenic multi-drug resistant STEC serogroups that can constitute public health hazard.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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