Number and Brightness Analysis: Visualization of Protein Oligomeric State in Living Cells.
| Autor: | Fukushima R; Laboratory of Molecular Cell Dynamics, Graduate School of Life Science, Hokkaido University, Sapporo, Japan., Yamamoto J; Bioimaging Research Group, Health and Medical Research Institute, The National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan., Kinjo M; Laboratory of Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan. kinjo@sci.hokudai.ac.jp. |
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| Jazyk: | angličtina |
| Zdroj: | Advances in experimental medicine and biology [Adv Exp Med Biol] 2021; Vol. 1310, pp. 31-58. |
| DOI: | 10.1007/978-981-33-6064-8_2 |
| Abstrakt: | Number and brightness (N&B) analysis helps to visualize protein oligomer and its localization in a living cell. N&B analysis provides apparent brightness, which reflects the oligomeric state of a fluorescently labeled protein, by analyzing the temporal intensity fluctuation at each pixel. N&B analysis is useful in understanding the dynamic oligomerization in signal transduction and neurodegenerative diseases. Furthermore, it also helps in gaining useful insights regarding the controlling mechanisms in protein function. In this chapter, we describe the basic theory and notations of N&B analysis implemented with confocal laser scanning microscopy for quantitative analyses. |
| Databáze: | MEDLINE |
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