Single-cell transcriptomics of human embryos identifies multiple sympathoblast lineages with potential implications for neuroblastoma origin.

Autor: Kameneva P; Department of Physiology and Pharmacology, Karolinska Institutet, Solna, Sweden., Artemov AV; Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Vienna, Austria., Kastriti ME; Department of Physiology and Pharmacology, Karolinska Institutet, Solna, Sweden.; Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Vienna, Austria.; Department of Molecular Neurosciences, Medical University of Vienna, Vienna, Austria., Faure L; Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Vienna, Austria., Olsen TK; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Otte J; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Erickson A; Department of Physiology and Pharmacology, Karolinska Institutet, Solna, Sweden., Semsch B; Department of Comparative Medicine, Karolinska Institutet, Solna, Sweden.; Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden., Andersson ER; Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden.; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden., Ratz M; Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden., Frisén J; Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden., Tischler AS; Department of Pathology and Laboratory Medicine, Tufts Medical Center, Boston, MA, USA., de Krijger RR; Princess Máxima Center for Pediatric Oncology CS, Utrecht, the Netherlands.; Deptartment of Pathology, University Medical Center Utrecht CX, Utrecht, the Netherlands., Bouderlique T; Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Vienna, Austria., Akkuratova N; Department of Physiology and Pharmacology, Karolinska Institutet, Solna, Sweden.; Institute of Translational Biomedicine, St. Petersburg University, St. Petersburg, Russia., Vorontsova M; Endocrinology Research Centre, Moscow, Russian Federation.; Moscow Institute of Physics and Technology, Dolgoprudniy, Russian Federation.; Institute for Regenerative Medicine, Lomonosov Moscow State University, Moscow, Russian Federation., Gusev O; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation.; RIKEN Innovation Center, RIKEN, Yokohama, Japan.; Center for Life Science Technologies, RIKEN, Yokohama, Japan., Fried K; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden., Sundström E; Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Solna, Sweden., Mei S; Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA., Kogner P; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Baryawno N; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Kharchenko PV; Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. peter_kharchenko@hms.harvard.edu.; Harvard Stem Cell Institute, Cambridge, MA, USA. peter_kharchenko@hms.harvard.edu., Adameyko I; Department of Physiology and Pharmacology, Karolinska Institutet, Solna, Sweden. igor.adameyko@meduniwien.ac.at.; Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Vienna, Austria. igor.adameyko@meduniwien.ac.at.
Jazyk: angličtina
Zdroj: Nature genetics [Nat Genet] 2021 May; Vol. 53 (5), pp. 694-706. Date of Electronic Publication: 2021 Apr 08.
DOI: 10.1038/s41588-021-00818-x
Abstrakt: Characterization of the progression of cellular states during human embryogenesis can provide insights into the origin of pediatric diseases. We examined the transcriptional states of neural crest- and mesoderm-derived lineages differentiating into adrenal glands, kidneys, endothelium and hematopoietic tissue between post-conception weeks 6 and 14 of human development. Our results reveal transitions connecting the intermediate mesoderm and progenitors of organ primordia, the hematopoietic system and endothelial subtypes. Unexpectedly, by using a combination of single-cell transcriptomics and lineage tracing, we found that intra-adrenal sympathoblasts at that stage are directly derived from nerve-associated Schwann cell precursors, similarly to local chromaffin cells, whereas the majority of extra-adrenal sympathoblasts arise from the migratory neural crest. In humans, this process persists during several weeks of development within the large intra-adrenal ganglia-like structures, which may also serve as reservoirs of originating cells in neuroblastoma.
Databáze: MEDLINE
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