Monitoring the infection process of Rhizopus stolonifer on strawberry fruit during storage using films based on chitosan/polyvinyl alcohol/polyvinylpyrrolidone and plant extracts.

Autor: Ventura-Aguilar RI; CONACYT-Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla km 6, San Isidro, Yautepec, Morelos 62731, Mexico. Electronic address: riventuraag@conacyt.mx., Díaz-Galindo EP; Facultad de Química, Universidad Autónoma del Estado de México, km 115 Car, Toluca-Ixtlahuaca, El Cerillo Piedras Blancas, Toluca 50295, Mexico., Bautista-Baños S; Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla km 6, San Isidro, Yautepec, Morelos 62731, Mexico., Mendoza-Acevedo S; Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional, Av. Luis Enrique Erro S/N, Zacatenco, Ciudad de México 07738, Mexico., Munguía-Cervantes JE; Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional, Av. Luis Enrique Erro S/N, Zacatenco, Ciudad de México 07738, Mexico., Correa-Pacheco ZN; CONACYT-Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla km 6, San Isidro, Yautepec, Morelos 62731, Mexico., Bosquez-Molina E; Biotechnology Department, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco 186, Colonia Vicentina, Mexico City, 09340, Mexico.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2021 Jul 01; Vol. 182, pp. 583-594. Date of Electronic Publication: 2021 Apr 06.
DOI: 10.1016/j.ijbiomac.2021.03.187
Abstrakt: Different formulations based on nanoparticles of chitosan-plant extracts were evaluated to detect the infection process from the earliest stage of the fungus Rhizopus stolonifer on strawberry fruit during storage. Chitosan/polyvinyl alcohol (Ch/PVA) and chitosan/polyvinylpyrrolidone (Ch/PVP) films enriched with nanoparticles (NPs) of chitosan blended with plant extracts were prepared. They were placed inside a plastic package containing inoculated fruits and stored at 25 °C for 72 h. The thickness values of the films were in the range of 0.10 to 0.25 mm. All samples showed a maximum absorbance peak of about 300-320 nm; however, the Ch/PVP films enriched with NPs of chitosan and 10% of radish extract had an evident decrease in the optical absorbance as the fungal infection progressed. Additionally, as observed by scanning electron microscopy, the cross-section and surface morphology of films were not modified during storage, and the growth of R. stolonifer was evident after 48 h. Therefore, the Ch/PVP films enriched with chitosan NPs blended with 10% radish extract could be a reliable indicator of this fungus's growth.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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