Role of Electrostatic Hotspots in the Selectivity of Complement Control Proteins Toward Human and Bovine Complement Inhibition.

Autor:	Narkhede YB; Department of Bioengineering, University of California, Riverside, CA., Gautam AK; National Centre for Cell Science, Pune University Campus, Ganeshkhind, India., Hsu RV; Department of Bioengineering, University of California, Riverside, CA., Rodriguez W; Department of Bioengineering, University of California, Riverside, CA., Zewde NT; Department of Bioengineering, University of California, Riverside, CA., Harrison RES; Department of Bioengineering, University of California, Riverside, CA., Arantes PR; Department of Bioengineering, University of California, Riverside, CA., Gaieb Z; Department of Bioengineering, University of California, Riverside, CA., Gorham RD Jr; Department of Bioengineering, University of California, Riverside, CA., Kieslich C; Department of Bioengineering, University of California, Riverside, CA.; Department of Chemical Engineering, Auburn University, Auburn, AL., Morikis D; Department of Bioengineering, University of California, Riverside, CA., Sahu A; National Centre for Cell Science, Pune University Campus, Ganeshkhind, India., Palermo G; Department of Bioengineering, University of California, Riverside, CA.; Department of Chemistry, University of California, Riverside, CA.
Jazyk:	angličtina
Zdroj:	Frontiers in molecular biosciences [Front Mol Biosci] 2021 Mar 16; Vol. 8, pp. 618068. Date of Electronic Publication: 2021 Mar 16 (Print Publication: 2021).
DOI:	10.3389/fmolb.2021.618068
Abstrakt:	Poxviruses are dangerous pathogens, which can cause fatal infection in unvaccinated individuals. The causative agent of smallpox in humans, variola virus , is closely related to the bovine vaccinia virus , yet the molecular basis of their selectivity is currently incompletely understood. Here, we examine the role of the electrostatics in the selectivity of the smallpox protein SPICE and vaccinia protein VCP toward the human and bovine complement protein C3b, a key component of the complement immune response. Electrostatic calculations, in-silico alanine-scan and electrostatic hotspot analysis, as introduced by Kieslich and Morikis ( PLoS Comput. Biol . 2012), are used to assess the electrostatic complementarity and to identify sites resistant to local perturbation where the electrostatic potential is likely to be evolutionary conserved. The calculations suggest that the bovine C3b is electrostatically prone to selectively bind its VCP ligand. On the other hand, the human isoform of C3b exhibits a lower electrostatic complementarity toward its SPICE ligand. Yet, the human C3b displays a highly preserved electrostatic core, which suggests that this isoform could be less selective in binding different ligands like SPICE and the human Factor H. This is supported by experimental cofactor activity assays revealing that the human C3b is prone to bind both SPICE and Factor H, which exhibit diverse electrostatic properties. Additional investigations considering mutants of SPICE and VCP that revert their selectivity reveal an "electrostatic switch" into the central modules of the ligands, supporting the critical role of the electrostatics in the selectivity. Taken together, these evidences provide insights into the selectivity mechanism of the complement regulator proteins encoded by the variola and vaccinia viruses to circumvent the complement immunity and exert their pathogenic action. These fundamental aspects are valuable for the development of novel vaccines and therapeutic strategies. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Narkhede, Gautam, Hsu, Rodriguez, Zewde, Harrison, Arantes, Gaieb, Gorham, Kieslich, Morikis, Sahu and Palermo.)
Databáze:	MEDLINE
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