Using egg yolk in a TRIS-Equex STM paste extender for freezing of dog semen is superior to egg yolk plasma, also after addition of lecithin and catalase.

Autor: Schäfer-Somi S; Department of Small Animals and Horses, Platform for AI and ET, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna, Austria. Electronic address: sabine.schaefer@vetmeduni.ac.at., Binder C; Clinic for Obstetrics, Gynecology and Andrology, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna, Austria. Electronic address: claudia.binder@vetmeduni.ac.at., Burak J; Platform for AI and ET, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna, Austria. Electronic address: jessica.burak@adacta.at., Papadopoulos N; Platform for AI and ET, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna, Austria. Electronic address: natipapadopoulou@gmail.com., Ilas J; Takeda Pharma Austria, Vienna, Austria. Electronic address: josenatoilas@gmail.com., Boersma A; Institute of In-vivo and In-vitro Models, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna, Austria. Electronic address: auke.boersma@vetmeduni.ac.at., Aurich C; Platform for AI and ET, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna, Austria. Electronic address: christine.aurich@vetmeduni.ac.at.
Jazyk: angličtina
Zdroj: Cryobiology [Cryobiology] 2021 Jun; Vol. 100, pp. 63-71. Date of Electronic Publication: 2021 Apr 04.
DOI: 10.1016/j.cryobiol.2021.03.009
Abstrakt: We compared the results of using egg yolk plasma (EYP) instead of egg yolk (EY) in a TRIS-based Equex STM Paste freezing extender system for dog semen [25]. We also tested whether the addition of lecithin and catalase to the EYP extenders would improve results. Fractionated semen collection was done in 17 stud dogs and the sperm rich fraction diluted with different extenders in 2 steps: (I) TRIS-fructose-citric acid extender (TRIS) containing 20% egg yolk (EY) and 3% glycerol [25], (II) TRIS containing 20% egg yolk plasma (EYP) and 3% glycerol, and (III) TRIS containing 20% EYP and 0.8% lecithin (EYP-L) and 3% glycerol. After equilibration the second dilution step was done: samples with (I) were diluted with TRIS-EY with 7% glycerol and 1% Equex STM paste [25]; samples with (II) and (III) were divided in 2 aliquots each, and one part diluted with TRIS-EYP or TRIS-EYP-L, both containing 7% glycerol and 1% Equex STM paste, and the other one part with the same extenders containing additionally 300 I.U./mL catalase. After freezing and thawing, samples were analyzed by CASA and sperm chromatin structure assay (SCSA); reactive oxygen species (ROS), degree of apoptosis and zona binding ability were determined. Semen samples with TRIS-EY with a final concentration of 5% glycerol and 0.5% Equex STM paste [25] showed best post thaw progressive motility (P), most intact cells, lowest percentage of ROS, acrosome damages, dead and apoptotic cells. Curvilinear velocity (VCL), DNA fragmentation, morphological abnormalities and zona binding ability did not differ between groups. Replacement of egg yolk by EYP increased the ROS and late apoptotic cells. Addition of lecithin and catalase to EYP containing extenders decreased motility and increased complete apoptosis. We conclude that egg yolk is superior to EYP in the here investigated extenders. The TRIS-based extender [25] with EYP could not be improved by addition of lecithin and catalase; however, in-vivo fertilization capacity of the here examined extenders remains to be investigated.
(Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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