| Autor: |
Chen Z; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China.; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, P. R. China., Lu Q; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China.; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, P. R. China., Liang Y; Mammalian Nutrition Physiology Genomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, Illinois 61801, United States., Cui X; Laboratory of Animal Developmental Biology, Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea., Wang X; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China., Mao Y; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China.; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, P. R. China., Yang Z; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China.; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, P. R. China. |
| Abstrakt: |
The quality of milk is inseparable from its milk components, and fatty acid content is a key factor affecting the quality of milk. In this study, the miRNA and mRNA profiles of the bovine mammary gland tissue during the dry period and the peak lactation period were determined through high-throughput sequencing. In total, 72 miRNA-mRNA regulatory pathways were screened, including miR-128/ PPARGC1A regulatory pathways. miR-128 can directly target PPARGC1A and inhibit its expression. In addition, the study also observed that there was a miR-128 binding site in the sequence of the circular RNA circ11103, and circ11103 significantly reduced the expression of miR-128. circ11103 upregulated the triglyceride levels in bovine mammary epithelial cells (BMECs) and increased the contents of unsaturated fatty acids. However, miR-128 decreased triglyceride and cholesterol levels in BMECs. This study aims to analyze the mechanism governing the regulatory effect of circ11103 on milk fat metabolism, which provides new insights into improving milk quality. |
