Changes in myocardial myosin heavy chain isoform composition with exercise and post-exercise cold-water immersion.

Autor: Al-Horani RA; Department of Exercise Science, Yarmouk University, Irbid, Jordan. raalhorani@yu.edu.jo., Mohammad MA; Department of Physiology, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan., Haifawi S; Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid, Jordan., Ihsan M; Research and Scientific Support, Aspetar Orthopedic and Sports Medicine Hospital, Doha, Qatar.
Jazyk: angličtina
Zdroj: Journal of muscle research and cell motility [J Muscle Res Cell Motil] 2021 Jun; Vol. 42 (2), pp. 183-191. Date of Electronic Publication: 2021 Apr 07.
DOI: 10.1007/s10974-021-09603-z
Abstrakt: This study investigated the changes in myocardial myosin heavy chain (MHC) isoforms, MHC-α and MHC-β composition in young healthy rodents following endurance training, with and without post-exercise cold-water immersion (CWI). Male rats were either trained on a treadmill for 10 weeks with (CWI) or without (Ex) regular CWI after each running session, or left sedentary (CON). Left ventricular mRNA of MHC-α, MHC-β, thyroid receptor α1 (TR-α1) and β (TR-β) were analyzed using rt-PCR and semiquantitative PCR analysis. MHC isoform protein composition was determined using SDS-PAGE electrophoresis. MHC-α isoform protein was predominant in all groups. The relative expression of MHC-β (%MHC-β) protein was not different between groups (CWI 34.7 ± 6.9%; Ex 32 ± 5.3%; CON 35.5 ± 10%; P = 0.7). MHC-β mRNA was reduced in Ex (0.7 ± 0.3-fold) compared to CWI (1.3 ± 0.2-fold; P < 0.001) and CON (1.01 ± 0.2-fold; P = 0.03). TRα1 mRNA was lower in CWI (0.4 ± 0.05-fold) than Ex (1.02 ± 0.3-fold) and CON (1.01 ± 0.2-fold) (P < 0.001 for both). CWI exhibited greater %MHC-β mRNA (56.8 ± 4.1%) than Ex (44.4 ± 7.7%; P = 0.001) and CON (48.5 ± 7.8%; P = 0.03). Neither exercise nor post-exercise CWI demonstrated a distinct effect on myocardial MHC protein isoform composition. However, CWI increased the relative expression of MHC-β mRNA compared with Ex and CON. Although this implicates a potential negative long-term impact of post-exercise CWI, future studies should include measures of cardiac function to better understand the effect of such isoform mRNA shifts following regular use of CWI.
(© 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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