Hematopoietic stem progenitor cells lacking HLA differ from those lacking GPI-anchored proteins in the hierarchical stage and sensitivity to immune attack in patients with acquired aplastic anemia.

Autor: Yoroidaka T; Department of Hematology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan., Hosokawa K; Department of Hematology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan., Imi T; Department of Hematology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan., Mizumaki H; Department of Hematology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan., Katagiri T; Clinical Laboratory Sciences, Kanazawa University Graduate School, Kanazawa, Japan., Ishiyama K; Department of Hematology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan., Yamazaki H; Division of Transfusion Medicine, Kanazawa University Hospital, Kanazawa, Japan., Azuma F; HLA laboratory, Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Tokyo, Japan., Nanya Y; Department of Pathology and Tumor Biology, Kyoto University, Kyoto, Japan., Ogawa S; Department of Pathology and Tumor Biology, Kyoto University, Kyoto, Japan., Nakao S; Department of Hematology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan. snakao8205@staff.kanazawa-u.ac.jp.
Jazyk: angličtina
Zdroj: Leukemia [Leukemia] 2021 Nov; Vol. 35 (11), pp. 3257-3267. Date of Electronic Publication: 2021 Apr 06.
DOI: 10.1038/s41375-021-01202-8
Abstrakt: To characterize glycosylphosphatidylinositol-anchored protein-deficient (GPI[-]) and HLA-class I allele-lacking (HLA[-]) hematopoietic stem progenitor cells (HSPCs) in acquired aplastic anemia (AA), we studied the peripheral blood (PB) of 56 AA patients in remission who possessed both (n = 13, Group A) or either GPI(-) (n = 34, Group B) and HLA(-) (n = 9, Group C) cell populations. Seventy-seven percent (10/13) of Group A had HLA(-) cells in all lineages of PB cells, including platelets, while only 23% (3/13) had GPI(-) cells in all lineages, and the median percentage of HLA(-) granulocytes in the total granulocytes (21.2%) was significantly higher than that of GPI(-) granulocytes (0.28%, P < 0.05). The greater lineage diversity in HLA(-) cells than in GPI(-) cells was also seen when Group B and Group C were compared. Longitudinal studies of seven patients in Group A showed a gradual decrease in the percentage of HLA(-) granulocytes, with a reciprocal increase in the GPI(-) granulocytes in four patients responding to cyclosporine (CsA) and an increase in the HLA(-) granulocytes with a stable or declining GPI(-) granulocytes in three patients in sustained remission off CsA therapy. These findings suggest that HLA(-) HSPCs differ from GPI(-) HSPCs in the hierarchical stage and sensitivity to immune attack in AA.
(© 2021. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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