A novel platform for discovery of differentially expressed microRNAs in patients with repeated implantation failure.

Autor:	Chen CH; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan; Department of Obstetrics and Gynecology, Ton Yen General Hospital, Hsinchu, Taiwan; Taiwan IVF Group Center for Reproductive Medicine and Infertility, Hsinchu, Taiwan., Lu F; Department of Obstetrics and Gynecology, Ton Yen General Hospital, Hsinchu, Taiwan; Taiwan IVF Group Center for Reproductive Medicine and Infertility, Hsinchu, Taiwan., Yang WJ; Department of Obstetrics and Gynecology, Ton Yen General Hospital, Hsinchu, Taiwan; Taiwan IVF Group Center for Reproductive Medicine and Infertility, Hsinchu, Taiwan., Yang PE; Quark Biosciences, Inc., Hsinchu, Taiwan., Chen WM; Quark Biosciences, Inc., Hsinchu, Taiwan., Kang ST; Quark Biosciences, Inc., Hsinchu, Taiwan., Huang YS; Quark Biosciences, Inc., Hsinchu, Taiwan., Kao YC; Quark Biosciences, Inc., Hsinchu, Taiwan., Feng CT; Quark Biosciences, Inc., Hsinchu, Taiwan., Chang PC; Quark Biosciences, Inc., Hsinchu, Taiwan., Wang T; Quark Biosciences, Inc., Hsinchu, Taiwan., Hsieh CA; Taiwan IVF Group Center for Reproductive Medicine and Infertility, Hsinchu, Taiwan., Lin YC; Taiwan IVF Group Center for Reproductive Medicine and Infertility, Hsinchu, Taiwan., Jen Huang JY; Department of Obstetrics and Gynecology, Ton Yen General Hospital, Hsinchu, Taiwan; Taiwan IVF Group Center for Reproductive Medicine and Infertility, Hsinchu, Taiwan; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Stanford University, Stanford, California., Wang LH; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan; Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan. Electronic address: lilywang@life.nthu.edu.tw.
Jazyk:	angličtina
Zdroj:	Fertility and sterility [Fertil Steril] 2021 Jul; Vol. 116 (1), pp. 181-188. Date of Electronic Publication: 2021 Apr 03.
DOI:	10.1016/j.fertnstert.2021.01.055
Abstrakt:	Objective: To identify predictor microRNAs (miRNAs) from patients with repeated implantation failure (RIF). Design: Systemic analysis of miRNA profiles from the endometrium of patients undergoing in vitro fertilization (IVF). Setting: University research institute, private IVF center, and molecular testing laboratory. Patient(s): Twenty five infertile patients in the discovery cohort and 11 patients in the validation cohort. Interventions(s): None. Main Outcome Measure(s): A signature set of miRNA associated with the risk of RIF. Result(s): We designed a reproductive disease-related PanelChip to access endometrium miRNA profiles in patients undergoing IVF. Three major miRNA signatures, including hsa-miR-20b-5p, hsa-miR-155-5p, and hsa-miR-718, were identified using infinite combination signature search algorithm analysis from 25 patients in the discovery cohort undergoing IVF. These miRNAs were used as biomarkers in the validation cohort of 11 patients. Finally, the 3-miRNA signature was capable of predicting patients with RIF with an accuracy >90%. Conclusion(s): Our findings indicated that specific endometrial miRNAs can be applied as diagnostic biomarkers to predict RIF. Such information will definitely help to increase the success rate of implantation practice. (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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