Ligand sensing enhances bacterial flagellar motor output via stator recruitment.

Autor:	Naaz F; Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India., Agrawal M; Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India., Chakraborty S; Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India., Tirumkudulu MS; Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India., Venkatesh KV; Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Apr 06; Vol. 10. Date of Electronic Publication: 2021 Apr 06.
DOI:	10.7554/eLife.62848
Abstrakt:	It is well known that flagellated bacteria, such as Escherichia coli , sense chemicals in their environment by a chemoreceptor and relay the signals via a well-characterized signaling pathway to the flagellar motor. It is widely accepted that the signals change the rotation bias of the motor without influencing the motor speed. Here, we present results to the contrary and show that the bacteria is also capable of modulating motor speed on merely sensing a ligand. Step changes in concentration of non-metabolizable ligand cause temporary recruitment of stator units leading to a momentary increase in motor speeds. For metabolizable ligand, the combined effect of sensing and metabolism leads to higher motor speeds for longer durations. Experiments performed with mutant strains delineate the role of metabolism and sensing in the modulation of motor speed and show how speed changes along with changes in bias can significantly enhance response to changes in its environment. Competing Interests: FN, MA, SC, MT, KV No competing interests declared (© 2021, Naaz et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu