New Insight into Vitamins E and K 1 as Anti-Quorum-Sensing Agents against Pseudomonas aeruginosa.

Autor: Soltani S; Department of Microbiology, Faculty of Science, University of Mazandaran, Babolsar, Iran., Fazly Bazzaz BS; Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.; Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran., Hadizadeh F; Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran., Roodbari F; Department of Microbiology, Faculty of Science, University of Mazandaran, Babolsar, Iran., Soheili V; Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Soheiliv@mums.ac.ir.
Jazyk: angličtina
Zdroj: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2021 May 18; Vol. 65 (6). Date of Electronic Publication: 2021 May 18 (Print Publication: 2021).
DOI: 10.1128/AAC.01342-20
Abstrakt: Today, antivirulence compounds that attenuate bacterial pathogenicity and have no interference with bacterial viability or growth are introduced as the next generation of antibacterial agents. However, the development of such compounds that can be used by humans is restricted by various factors, including the need for extensive economic investments, the inability of many molecules to penetrate the membrane of Gram-negative bacteria, and unfavorable pharmacological properties and cytotoxicity. Here, we take a new and different look into two frequent supplements, vitamin E and K 1 , as anti-quorum-sensing agents against Pseudomonas aeruginosa , a pathogen that is hazardous to human life and responsible for several diseases. Both vitamins showed significant anti-biofilm activity (62% and 40.3% reduction by vitamin E and K 1 , respectively), and the expression of virulence factors, including pyocyanin, pyoverdine, and protease, was significantly inhibited, especially in the presence of vitamin E. Cotreatment of constructed biofilms with these vitamins plus tobramycin significantly reduced the number of bacterial cells sheltered inside the impermeable matrix (71.6% and 69% by a combination of tobramycin and vitamin E or K 1 , respectively). The in silico studies, besides the similarities of chemical structures, reinforce the possibility that both vitamins act through inhibition of the PqsR protein. This is the first report of the antivirulence and antipathogenic activity of vitamin E and K 1 against P. aeruginosa and confirms their potential for further research against other multidrug-resistant bacteria.
(Copyright © 2021 American Society for Microbiology.)
Databáze: MEDLINE
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