| Autor: |
Mitrović I; Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia., Grahovac J; Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia., Hrustić J; Institute of Pesticides and Environmental Protection, Beograd-Zemun, Serbia., Jokić A; Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia., Dodić J; Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia., Mihajlović M; Institute of Pesticides and Environmental Protection, Beograd-Zemun, Serbia., Grahovac M; Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia. |
| Jazyk: |
angličtina |
| Zdroj: |
Environmental technology [Environ Technol] 2022 Aug; Vol. 43 (19), pp. 3000-3013. Date of Electronic Publication: 2021 Apr 18. |
| DOI: |
10.1080/09593330.2021.1913241 |
| Abstrakt: |
Search for more environment-friendly methods for controlling plant diseases that would contribute to the goal of sustainability in agriculture is in focus. In the present study, the potential of Streptomyces hygroscopicus isolated from soil sample in the production of biocontrol agents, nigericin and niphimycin, effective against Alternaria alternata storage apple pathogen was examined. Also, modelling and optimization of medium composition for biocontrol agent biosynthesis was performed. The results showed that the optimum amount of C 3 H 8 O 3 , (NH 4 ) 2 SO 4 and K 2 HPO 4 in the medium for Streptomyces hygroscopicus biosynthesis is 20, 0.25 and 1.46 g/L, respectively. Scale-up and validation of the obtained results performed in the 3 L laboratory-scale bioreactor showed that on the optimized medium at an aeration rate of 0.7 vvm and an agitation speed of 200 rpm, produced nigericin and niphimycin, showed high activity. Under the same conditions, cultivation of S. hygroscopicus was performed in a 7 L laboratory bioreactor in a medium with waste glycerol instead of pure glycerol. Results showed that the methanol extract of S. hygroscopicus cultivation liquid, containing nigericin and niphimycin, was high effective against two Alternaria isolates. This was confirmed in vitro by obtaining large inhibition zone diameters on A. alternata KA10 (47 mm) and T1Jg 3 (44.33 mm) isolates. After successful in vitro analysis, in planta testing was performed. It was found that necrosis diameters that were measured on artificially inoculated apple fruits with A. alternata compared to necrosis diameter measured on untreated, control fruits, were 4.47 and 3.56 times smaller. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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