Bio-functionalized titanium surfaces with modified silk fibroin carrying titanium binding motif to enhance the ossific differentiation of MC3T3-E1.

Autor: Watanabe M; Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Koto, Tokyo, Japan.; Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan., Bhawal UK; Department of Biochemistry and Molecular Biology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan., Takemoto S; Department of Biomedical Engineering, Iwate Medical University, Yahaba, Iwate, Japan., Nishiyama N; Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan., Nakahara Y; Transgenic Silkworm Research Unit, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan., Tatematsu KI; Transgenic Silkworm Research Unit, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan., Sezutsu H; Transgenic Silkworm Research Unit, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan., Kuwabara N; Gunma Sericultural Technology Center, Maebashi, Gunma, Japan., Minamisawa T; Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Koto, Tokyo, Japan., Shiba K; Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Koto, Tokyo, Japan., Asakura T; Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Jul; Vol. 118 (7), pp. 2585-2596. Date of Electronic Publication: 2021 Apr 13.
DOI: 10.1002/bit.27777
Abstrakt: Silk fibroin (SF) from Bombyx mori has superior properties as both a textile and a biomaterial, and has been used to functionalize the surfaces of various medical inorganic materials including titanium (Ti). In this study, we endowed SF with reversible binding ability to Ti by embedding a titanium binding motif (minTBP-1 and RKLPDA). Artificial SF proteins were first created by conjugating gene cassettes for SF motif (AGSGAG) and minTBP-1 motif with different ratios, which have been shown to bind reversibly to Ti surfaces in quartz crystal microbalance analyses. Based on these results, the functionalized SF (TiBP-SF) containing the designed peptide [TS[(AGSGAG) 3 AS] 2 RKLPDAS] 8 was prepared from the cocoon of transgenic B. mori, which accelerates the ossific differentiation of MC3T3-E1 cells when coated on titanium substrates. Thus, TiBP-SF presents an alternative for endowing the surfaces of titanium materials with osseointegration functionality, which would allow the exploration of potential applications in the medical field.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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