Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH.

Autor: Castillo YM; Department of Marine Biology and Oceanography, Institute of Marine Sciences (CSIC), Barcelona, Spain., Forn I; Department of Marine Biology and Oceanography, Institute of Marine Sciences (CSIC), Barcelona, Spain., Yau S; Department of Marine Biology and Oceanography, Institute of Marine Sciences (CSIC), Barcelona, Spain., Morán XAG; Red Sea Research Center, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia., Alonso-Sáez L; Centro Oceanográfico de Gijón/Xixón, IEO, Gijón/Xixón, Spain.; AZTI Marine Research, Basque Research and Technology Alliance (BRTA), Txatxarramendi ugartea z/g, Sukarrieta, Spain., Arandia-Gorostidi N; Centro Oceanográfico de Gijón/Xixón, IEO, Gijón/Xixón, Spain.; Department of Earth System Science, Stanford University, Stanford, CA, USA., Vaqué D; Department of Marine Biology and Oceanography, Institute of Marine Sciences (CSIC), Barcelona, Spain., Sebastián M; Department of Marine Biology and Oceanography, Institute of Marine Sciences (CSIC), Barcelona, Spain.; Institute of Oceanography and Global Change (IOCAG), University of Las Palmas de Gran Canaria (ULPGC), Telde, Spain.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2021 Jun; Vol. 23 (6), pp. 3009-3019. Date of Electronic Publication: 2021 Apr 27.
DOI: 10.1111/1462-2920.15504
Abstrakt: Ostreococcus is a cosmopolitan marine genus of phytoplankton found in mesotrophic and oligotrophic waters, and the smallest free-living eukaryotes known to date, with a cell diameter close to 1 μm. Ostreococcus has been extensively studied as a model system to investigate viral-host dynamics in culture, yet the impact of viruses in naturally occurring populations is largely unknown. Here, we used Virus Fluorescence in situ Hybridization (VirusFISH) to visualize and quantify viral-host dynamics in natural populations of Ostreococcus during a seasonal cycle in the central Cantabrian Sea (Southern Bay of Biscay). Ostreococcus were predominantly found during summer and autumn at surface and 50 m depth, in coastal, mid-shelf and shelf waters, representing up to 21% of the picoeukaryotic communities. Viral infection was only detected in surface waters, and its impact was variable but highest from May to July and November to December, when up to half of the population was infected. Metatranscriptomic data available from the mid-shelf station unveiled that the Ostreococcus population was dominated by the species O. lucimarinus. This work represents a proof of concept that the VirusFISH technique can be used to quantify the impact of viruses on targeted populations of key microbes from complex natural communities.
(© 2021 Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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