Experimental Chronic Prostatitis/Chronic Pelvic Pain Syndrome Increases Anxiety-Like Behavior: The Role of Brain Oxidative Stress, Serum Corticosterone, and Hippocampal Parvalbumin-Positive Interneurons.

Autor: Šutulović N; Institute of Medical Physiology 'Richard Burian', Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia., Grubač Ž; Institute of Medical Physiology 'Richard Burian', Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia., Šuvakov S; Institute of Clinical and Medical Biochemistry, Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia., Jerotić D; Institute of Clinical and Medical Biochemistry, Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia., Puškaš N; Institute of Histology and Embryology 'Aleksandar Đ. Kostić', Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia., Macut D; Clinic of Endocrinology, Diabetes and Metabolic Disease, CCS, Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia., Rašić-Marković A; Institute of Medical Physiology 'Richard Burian', Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia., Simić T; Institute of Clinical and Medical Biochemistry, Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia., Stanojlović O; Institute of Medical Physiology 'Richard Burian', Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia., Hrnčić D; Institute of Medical Physiology 'Richard Burian', Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
Jazyk: angličtina
Zdroj: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2021 Mar 02; Vol. 2021, pp. 6687493. Date of Electronic Publication: 2021 Mar 02 (Print Publication: 2021).
DOI: 10.1155/2021/6687493
Abstrakt: Mechanisms of the brain-related comorbidities in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) are still largely unknown, although CP/CPPS is one of the major urological problems in middle-aged men, while these neuropsychological incapacities considerably diminish life quality. The objectives of this study were to assess behavioral patterns in rats with CP/CPPS and to determine whether these patterns depend on alterations in the brain oxidative stress, corticosterone, and hippocampal parvalbumin-positive (PV+) interneurons. Adult male Wistar albino rats from CP/CPPS (intraprostatic injection of 3% λ -carrageenan, day 0) and sham (0.9% NaCl) groups were subjected to pain and anxiety-like behavior tests (days 2, 3, and 7). Afterwards, rats were sacrificed and biochemical and immunohistochemical analyses were performed. Scrotal allodynia and prostatitis were proven in CP/CPPS, but not in sham rats. Ethological tests (open field, elevated plus maze, and light/dark tests) revealed significantly increased anxiety-like behavior in rats with CP/CPPS comparing to their sham-operated mates starting from day 3, and there were significant intercorrelations among parameters of these tests. Increased oxidative stress in the hippocampus, thalamus, and cerebral cortex, as well as increased serum corticosterone levels and decreased number of hippocampal PV+ neurons, was shown in CP/CPPS rats, compared to sham rats. Increased anxiety-like behavior in CP/CPPS rats was significantly correlated with these brain biochemical and hippocampal immunohistochemical alterations. Therefore, the potential mechanisms of observed behavioral alterations in CP/CPPS rats could be the result of an interplay between increased brain oxidative stress, elevated serum corticosterone level, and loss of hippocampal PV+ interneurons.
Competing Interests: The authors declare that they have no conflict of interest.
(Copyright © 2021 Nikola Šutulović et al.)
Databáze: MEDLINE
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