Impact of multigenerational exposure to AgNO 3 or NM300K Ag NPs on antioxidant defense and oxidative stress in Caenorhabditis elegans.

Autor: Rossbach LM; Norwegian University of Life Sciences, Faculty of Environmental Sciences and Natural Resource Management, P.O. BOX 5003 NMBU, No-1432 Ås, Norway. Electronic address: Lisa.rossbach@nmbu.no., Oughton DH; Norwegian University of Life Sciences, Faculty of Environmental Sciences and Natural Resource Management, P.O. BOX 5003 NMBU, No-1432 Ås, Norway., Maremonti E; Norwegian University of Life Sciences, Faculty of Environmental Sciences and Natural Resource Management, P.O. BOX 5003 NMBU, No-1432 Ås, Norway., Eide DM; Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway., Brede DA; Norwegian University of Life Sciences, Faculty of Environmental Sciences and Natural Resource Management, P.O. BOX 5003 NMBU, No-1432 Ås, Norway.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2021 Mar 31; Vol. 216, pp. 112178. Date of Electronic Publication: 2021 Mar 31.
DOI: 10.1016/j.ecoenv.2021.112178
Abstrakt: Adaptation of the nematode Caenorhabditis elegans towards NM300K silver nanoparticles (Ag NPs) has previously been demonstrated. In the current study, the sensitivity to a range of secondary stressors (CeO 2 NP, Ce 3+ , Cu 2+ , Cd 2+ , and Paraquat) following the multigenerational exposure to silver nanoparticles (Ag NPs NM300K) or AgNO 3 was investigated. This revealed improved tolerance to the ROS inducer Paraquat with higher fecundity after pre-exposure to Ag NP, indicating an involvement of reactive oxygen species (ROS) metabolism in the adaptive response to NM300K. The potential contribution of the antioxidant defenses related to adaptive responses was investigated across six generations of exposure using the sod-1::GFP reporter (GA508), and the Grx1-roGFP2 (GRX) biosensor strains. Results showed an increase in sod-1 expression by the F3 generation, accompanied by a reduction of GSSG/GSH ratios, from both AgNO 3 and Ag NP exposures. Continuous exposure to AgNO 3 and Ag NP until the F6 generation resulted in a decreased sod-1 expression, with a concomitant increase in GSSG/GSH ratios. The results thus show that despite an initial enhancement, the continuous exposure to Ag caused a severe impairment of the antioxidant defense capacity in C. elegans.
(Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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