Molecular characteristics of established trophoblast-derived cell lines.

Autor: Pastuschek J; Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747, Jena, Germany., Nonn O; Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 8010, Graz, Austria., Gutiérrez-Samudio RN; Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747, Jena, Germany., Murrieta-Coxca JM; Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747, Jena, Germany., Müller J; Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747, Jena, Germany., Sanft J; Institute for Forensic Medicine, Jena University Hospital, Jena, Germany., Huppertz B; Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 8010, Graz, Austria., Markert UR; Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747, Jena, Germany., Groten T; Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747, Jena, Germany. Electronic address: tanja.groten@med.uni-jena.de., Morales-Prieto DM; Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747, Jena, Germany. Electronic address: diana.morales@med.uni-jena.de.
Jazyk: angličtina
Zdroj: Placenta [Placenta] 2021 May; Vol. 108, pp. 122-133. Date of Electronic Publication: 2021 Mar 04.
DOI: 10.1016/j.placenta.2021.02.022
Abstrakt: Introduction: Research on human placental development and function lacks a conclusive in vivo model. To investigate the intracellular molecular mechanisms in trophoblast cells, different cell lines have been established during the last decades. So far, none of these accomplishes all features of primary trophoblast, thus their suitability as well as the transferability of the results has been discussed. The aim of this study is to assess molecular markers and features matching different trophoblast subpopulations in trophoblastic cell lines to provide orientation on their suitability and relevance for distinct research questions.
Methods: The commonly used trophoblastic cell lines, BeWo, JEG-3, HTR-8/SVneo, AC1-M59, AC1-M32, ACH-3P and Swan71 were selected. qPCR and immunoblotting were used to determine expression of characteristic molecular markers. C14MC, C19MC and miR-371-3 miRNA expression were investigated by real time PCR. Proliferation, migration and network stabilization assays were performed. Hormone secretion was determined by chemiluminescent-immunoassays. DNA profiles were obtained by Short Tandem Repeat (STR)-genotyping.
Results: Immortalized cell lines differ from choriocarcinoma-derived ones in the expression of HLA-G, E-cadherin, N-cadherin, VE-cadherin, cadherin-11, cytokeratin 7, vimentin, ADAM12 and PRG2. Compared to choriocarcinoma-derived cell lines, expression of C19MC and hormone secretion were almost absent in immortalized cell lines. Conversely, they express C14MC and exhibit higher migration and network stabilization.
Discussion: The data presented will help justify the use of a cell line to evaluate distinct features of trophoblast biology and pathology. In general, characteristics and markers of choriocarcinoma derived cell lines seem to be more similar to in vivo trophoblast than immortalized cell lines and thus might be regarded as more suitable models.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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