| Autor: |
Hernández-Gómez JG; Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu 399, UP Adolfo López Mateos, Mexico City CP 07738, Mexico., López-Bonilla A; Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec CP 62731, Mexico., Trejo-Tapia G; Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec CP 62731, Mexico., Ávila-Reyes SV; Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec CP 62731, Mexico., Jiménez-Aparicio AR; Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec CP 62731, Mexico., Hernández-Sánchez H; Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu 399, UP Adolfo López Mateos, Mexico City CP 07738, Mexico. |
| Abstrakt: |
Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The activity was assessed using a qualitative direct plate test and a quantitative high-performance thin- layer chromatography assay. The six strains differed in their BSH substrate preference and activity. Lactobacillus plantarum DGIA1, a potentially probiotic strain isolated from a double cream cheese from Chiapas, Mexico, showed excellent deconjugation activities in the four tested bile acids (69, 100, 81, and 92% for sodium glycocholate, glycodeoxycholate, taurocholate, and taurodeoxycholate, respectively). In the case of the commercial probiotic yeast Saccharomyces boulardii , the deconjugation activities were good against sodium glycodeoxycholate, taurocholate, and taurodeoxycholate (100, 57, and 63%, respectively). These last two results are part of the novelty of the work. A weak deconjugative activity (5%) was observed in the case of sodium glycocholate. This is the first time that the BSH activity has been detected in this yeast. |
