| Autor: |
Mendes YC; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís 65075-120, Brazil., Mesquita GP; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís 65075-120, Brazil., Costa GDE; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís 65075-120, Brazil., Barbosa da Silva AC; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís 65075-120, Brazil., Gouveia E; Department of Antibiotics, Federal University of Pernambuco, Cidade Universitária, Recife 50670-901, Brazil., Silva MRC; Laboratório de Química Ambiental, Universidade Ceuma, São Luís 65075-120, Brazil., Monteiro-Neto V; Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, São Luís 65085-582, Brazil., Miranda RCM; Laboratório de Microbiologia Ambiental, Universidade Ceuma, São Luís 65075-120, Brazil., Nascimento da Silva LC; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís 65075-120, Brazil., Zagmignan A; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís 65075-120, Brazil. |
| Abstrakt: |
Fruit juices have been emerging as excellent vehicles for development of probiotic products due to their nutritional properties and presence of bioactive compounds. This work evaluated the growth and viability of Limosilactobacillus fermentum ATCC 23271 and Lacticaseibacillus rhamnosus ATCC 9595 in bacuri juice ( Platonia insignis Mart., Clusiaceae). Both strains were able to grow in bacuri juice, without any supplementation. Viability was kept after 28 days of storage; however, growth was significantly higher for L. rhamnosus ATCC 9595 (7.40 ± 0.04 Log CFU/mL). Following this, the effects of bacterial inoculum and pulp concentration on growth and lactic acid production by L. rhamnosus ATCC 9595 were investigated using a central composite rotational design. The inoculum concentration was the main factor for obtaining the most favorable relation between growth and organic acid production (G/pH ratio). Among the tested conditions, those used in assay 6 allowed the best G/pH ratio (2.13) and higher lactic acid production (4.14 g/L). In these conditions, L. rhamnosus ATCC 9595 grown in bacuri juice showed the same resistance towards acidification or addition of lysozyme than when cultivated in MRS. Finally, the anti-infective effects of fermented and non-fermented juices were analyzed using Tenebrio molitor larvae infected by enteroaggregative Escherichia coli 042. The pre-treatment with supernatants of both fermented and non-fermented juices significantly increased the survival of E. coli- infected larvae. However, only the L. rhamnosus -fermented juice had protective effects when inoculated 2 h after infection. Collectively, the results obtained in this research allowed the basis for the development of a non-dairy probiotic product from bacuri juice. |
