| Autor: |
Pires FCS; LABEX (Extraction Laboratory), LABTECS (Supercritical Technology Laboratory), PPGCTA (PostGraduate Program in Food Science and Technology), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil., Oliveira JC; LABEX (Extraction Laboratory), FEA (College of Food Engineering), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil., Menezes EGO; LABEX (Extraction Laboratory), PRODERNA (Postgraduate Program in Natural Resources Engineering in the Amazon), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil., Silva APSE; LABEX (Extraction Laboratory), LABTECS (Supercritical Technology Laboratory), PPGCTA (PostGraduate Program in Food Science and Technology), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil., Ferreira MCR; LABEX (Extraction Laboratory), LABTECS (Supercritical Technology Laboratory), PPGCTA (PostGraduate Program in Food Science and Technology), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil., Siqueira LMM; LABEX (Extraction Laboratory), PRODERNA (Postgraduate Program in Natural Resources Engineering in the Amazon), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil., Almada-Vilhena AO; CEABIO (Center for Advanced Studies of the Biodiversity and Cell Culture Laboratory), PCT-Guamá (Guamá Science and Technology Park), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil., Pieczarka JC; CEABIO (Center for Advanced Studies of the Biodiversity and Cell Culture Laboratory), PCT-Guamá (Guamá Science and Technology Park), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil., Nagamachi CY; CEABIO (Center for Advanced Studies of the Biodiversity and Cell Culture Laboratory), PCT-Guamá (Guamá Science and Technology Park), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil., Carvalho Junior RN; LABEX (Extraction Laboratory), LABTECS (Supercritical Technology Laboratory), FEA (College of Food Engineering), ITEC (Institute of Technology), UFPA (Federal University of Pará), Augusto Corrêa Street S/N, Guamá, Belém, PA 66075-900, Brazil. |
| Abstrakt: |
The use of clean technologies in the development of bioactive plant extracts has been encouraged, but it is necessary to verify the cytotoxicity and cytoprotection for food and pharmaceutical applications. Therefore, the objective of this work was to obtain the experimental data of the supercritical sequential extraction of murici pulp, to determine the main bioactive compounds obtained and to evaluate the possible cytotoxicity and cytoprotection of the extracts in models of HepG2 cells treated with H 2 O 2 . The murici pulp was subjected to sequential extraction with supercritical CO 2 and CO 2 +ethanol, at 343.15 K, and 22, 32, and 49 MPa. Higher extraction yields were obtained at 49 MPa. The oil presented lutein (224.77 µg/g), oleic, palmitic, and linoleic, as the main fatty acids, and POLi (17.63%), POO (15.84%), PPO (13.63%), and LiOO (10.26%), as the main triglycerides. The ethanolic extract presented lutein (242.16 µg/g), phenolic compounds (20.63 mg GAE/g), and flavonoids (0.65 mg QE/g). The ethanolic extract showed greater antioxidant activity (122.61 and 17.14 µmol TE/g) than oil (43.48 and 6.04 µmol TE/g). Both extracts did not show cytotoxicity and only murici oil showed a cytoprotective effect. Despite this, the results qualify both extracts for food/pharmaceutical applications. |
