| Autor: |
Nasirzade J; Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria., Alccayhuaman KAA; Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria., Kargarpour Z; Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria., Kuchler U; Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria.; Department of Oral Surgery, Medical University of Vienna, 1090 Vienna, Austria., Strauss FJ; Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria.; Clinic of Reconstructive Dentistry, University of Zurich, 8032 Zurich, Switzerland.; Department of Conservative Dentistry, School of Dentistry, University of Chile, Santiago 1058, Chile., Panahipour L; Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria., Kampleitner C; Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Medical University of Vienna, 1090 Vienna, Austria.; Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, 1200 Vienna, Austria.; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria., Heimel P; Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Medical University of Vienna, 1090 Vienna, Austria.; Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, 1200 Vienna, Austria.; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria., Schwarz F; Department of Oral Surgery and Implantology, Johann Wolfgang Goethe-University, Carolinum, 60596 Frankfurt am Main, Germany., Gruber R; Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria.; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.; Department of Periodontology, School of Dental Medicine, University of Bern, 3012 Bern, Switzerland. |
| Abstrakt: |
Autogenous tooth roots are increasingly applied as a grafting material in alveolar bone augmentation. Since tooth roots undergo creeping substitution similar to bone grafts, it can be hypothesized that osteoclasts release the growth factors stored in the dentin thereby influencing bone formation. To test this hypothesis, collagen membranes were either soaked in acid dentin lysates (ADL) from extracted porcine teeth or serum-free medium followed by lyophilization. Thereafter, these membranes covered standardized 5-mm-diameter critical-size defects in calvarial bone on rats. After four weeks of healing, micro-computed tomography and histological analyses using undecalcified thin ground sections were performed. Micro-computed tomography of the inner 4.5 mm calvaria defects revealed a median bone defect coverage of 91% (CI: 87-95) in the ADL group and 94% (CI: 65-100) in the control group, without significant differences between the groups (intergroup p > 0.05). Furthermore, bone volume (BV) was similar between ADL group (5.7 mm 3 , CI: 3.4-7.1) and control group (5.7 mm 3 , CI: 2.9-9.7). Histomorphometry of the defect area confirmed these findings with bone area values amounting to 2.1 mm 2 (CI: 1.2-2.6) in the ADL group and 2.0 mm 2 (CI: 1.1-3.0) in the control group. Together, these data suggest that acid dentin lysate lyophilized onto collagen membranes failed to modulate the robust bone formation when placed onto calvarial defects. |
