| Autor: |
Reddy YM; Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, A.P., India., Kumar SPJ; Seed Biotechnology Laboratory, ICAR-Indian Institute of Seed Science, Mau 275103, Uttar Pradesh, India.; ICAR-Directorate of Floricultural Research, Pune 411005, Maharashtra, India., Saritha KV; Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, A.P., India., Gopal P; Electrochemical Research Laboratory, Department of Chemistry, Sri Venkateswara University, Tirupati 517502, A.P., India., Reddy TM; Electrochemical Research Laboratory, Department of Chemistry, Sri Venkateswara University, Tirupati 517502, A.P., India., Simal-Gandara J; Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science and Technology, Ourense Campus, University of Vigo, E32004 Ourense, Spain. |
| Abstrakt: |
Gardenia latifolia Ait. (Rubiaceae) is also known as Indian Boxwood is a small deciduous tree often growing in southern states of India. In the present study, phytochemical profiling of methanolic extract of G. latifolia fruits were carried out using FTIR and LC-MS/MS analysis. Besides, its antioxidant and antimicrobial potential have been analysed using DPPH activity, differential pulse voltammetry and resazurin microtiter assay, respectively. Phytochemical profiling revealed the presence of 22 major diversified compounds and main were 3-caffeoyl quinic acid (chlorogenic acid), 3,4-Di-O-caffeoyl quinic acid, 6-O-trans-feruloylgenipin gentiobioside, 10-(6-O-trans sinapoyl glucopyranosyl) gardendiol, isoquercitrin, scortechinones, secaubryenol, iridoids and quercetin 3-rutinoside (rutin). The extract showed antioxidant activity (IC 50 = 65.82) and powerful antibacterial activity with lowest minimum inhibitory concentration against Gram-positive Staphylococcus aureus (15.62 µg/µL), Bacillus subtilis (31.25 µg/µL) than gram negative Escherichia coli (62.5 µg/µL), Klebsiella pneumoniae (62.5 µg/µL), Pseudomonas aeruginosa (31.25 µg/µL). This study shows that the fruits of G. latifolia have tremendous potential to be used in food industries, phyto-therapeutics and cosmetic industries. |
