Genomic and Phenotypic Diversity of Cultivated and Wild Tomatoes with Varying Levels of Heat Tolerance.

Autor:	Ayenan MAT; West Africa Centre for Crop Improvement, College of Basic and Applied Science, University of Ghana, PMB LG30 Legon, Ghana.; World Vegetable Center, West and Central Africa-Coastal and Humid Regions, IITA-Benin Campus, 08 BP 0932 Cotonou, Benin., Danquah A; West Africa Centre for Crop Improvement, College of Basic and Applied Science, University of Ghana, PMB LG30 Legon, Ghana.; Department of Crop Science, Faculty of Agriculture, College of Basic and Applied Sciences, University of Ghana, P. O. Box LG44 Legon, Ghana., Agre PA; International Institute of Tropical Agriculture (IITA), PMB 5320, Ibadan 200001, Nigeria., Hanson P; World Vegetable Center, West and Central Africa-Coastal and Humid Regions, IITA-Benin Campus, 08 BP 0932 Cotonou, Benin., Asante IK; West Africa Centre for Crop Improvement, College of Basic and Applied Science, University of Ghana, PMB LG30 Legon, Ghana.; Department of Plant Biology and Environmental Science, College of Basic and Applied Sciences, University of Ghana, P. O. Box LG55 Legon, Ghana., Danquah EY; West Africa Centre for Crop Improvement, College of Basic and Applied Science, University of Ghana, PMB LG30 Legon, Ghana.; Department of Crop Science, Faculty of Agriculture, College of Basic and Applied Sciences, University of Ghana, P. O. Box LG44 Legon, Ghana.
Jazyk:	angličtina
Zdroj:	Genes [Genes (Basel)] 2021 Mar 29; Vol. 12 (4). Date of Electronic Publication: 2021 Mar 29.
DOI:	10.3390/genes12040503
Abstrakt:	Assessment of genetic variability in heat-tolerant tomato germplasm is a pre-requisite to improve yield and fruit quality under heat stress. We assessed the population structure and diversity in a panel of three Solanum pimpinellifolium (wild tomatoes) and 42 S. lycopersicum (cultivated tomatoes) lines and accessions with varying heat tolerance levels. The DArTseq marker was used for the sequencing and 5270 informative single nucleotide polymorphism (SNP) markers were retained for the genomic analysis. The germplasm was evaluated under two heat stress environments for five yield and flower related traits. The phenotypic evaluation revealed moderate broad-sense heritabilities for fruit weight per plant and high broad-sense heritabilities for fruit weight, number of inflorescences per plant, and number of flowers per inflorescence. The hierarchical clustering based on identity by state dissimilarity matrix and UPGMA grouped the germplasm into three clusters. The cluster analysis based on heat-tolerance traits separated the germplasm collection into five clusters. The correlation between the phenotypic and genomic-based distance matrices was low ( r = 0.2, p < 0.05). The joint phenotypic and genomic-based clustering grouped the germplasm collection into five clusters well defined for their response to heat stress ranging from highly sensitive to highly tolerant groups. The heat-sensitive and heat-tolerant clusters of S. lycopersicum lines were differentiated by a specific pattern of minor allele frequency distribution on chromosome 11. The joint phenotypic and genomic analysis revealed important diversity within the germplasm collection. This study provides the basis for efficient selection of parental lines to breed heat-tolerant varieties.
Databáze:	MEDLINE
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