| Autor: |
Mohanraj U; Department of Virology, University of Helsinki, 00290 Helsinki, Finland., Jokinen M; Department of Virology, University of Helsinki, 00290 Helsinki, Finland., Thapa RR; Department of Virology, University of Helsinki, 00290 Helsinki, Finland., Paloniemi M; Faculty of Medicine and Health Technology, Tampere University, 33100 Tampere, Finland., Vesikari T; Nordic Research Network Oy, 33700 Tampere, Finland., Lappalainen M; Helsinki University Hospital Laboratory (HUSLAB), 00290 Helsinki, Finland., Tarkka E; Helsinki University Hospital Laboratory (HUSLAB), 00290 Helsinki, Finland., Nora-Krūkle Z; Institute of Microbiology and Virology, Rīga Stradiņš University, 1067 Riga, Latvia., Vilmane A; Institute of Microbiology and Virology, Rīga Stradiņš University, 1067 Riga, Latvia., Vettenranta K; Helsinki University Hospital, 00280 Helsinki, Finland., Mangani C; College of Medicine, University of Malawi, Blantyre 3, Malawi., Oikarinen S; Faculty of Medicine and Health Technology, Tampere University, 33100 Tampere, Finland., Fan YM; Faculty of Medicine and Health Technology, Tampere University, 33100 Tampere, Finland., Ashorn P; Faculty of Medicine and Health Technology, Tampere University, 33100 Tampere, Finland., Väisänen E; Department of Virology, University of Helsinki, 00290 Helsinki, Finland., Söderlund-Venermo M; Department of Virology, University of Helsinki, 00290 Helsinki, Finland. |
| Abstrakt: |
Three human protoparvoviruses, bufavirus (BuV), tusavirus (TuV) and cutavirus (CuV), have recently been discovered in diarrheal stool. BuV has been associated with diarrhea and CuV with cutaneous T-cell lymphoma, but there are hardly any data for TuV or CuV in stool or respiratory samples. Hence, using qPCR and IgG enzyme immunoassays, we analyzed 1072 stool, 316 respiratory and 445 serum or plasma samples from 1098 patients with and without gastroenteritis (GE) or respiratory-tract infections (RTI) from Finland, Latvia and Malawi. The overall CuV-DNA prevalences in stool samples ranged between 0-6.1% among our six patient cohorts. In Finland, CuV DNA was significantly more prevalent in GE patients above rather than below 60 years of age (5.1% vs 0.2%). CuV DNA was more prevalent in stools among Latvian and Malawian children compared with Finnish children. In 10/11 CuV DNA-positive adults and 4/6 CuV DNA-positive children with GE, no known causal pathogens were detected. Interestingly, for the first time, CuV DNA was observed in two nasopharyngeal aspirates from children with RTI and the rare TuV in diarrheal stools of two adults. Our results provide new insights on the occurrence of human protoparvoviruses in GE and RTI in different countries. |
