| Autor: |
Boonphang O; Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand., Ontawong A; Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand., Pasachan T; Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand., Phatsara M; Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand., Duangjai A; Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand., Amornlerdpison D; Centre of Excellence in Agricultural Innovation for Graduate Entrepreneur and Faculty of Fisheries Technology and Aquatic Resources, Maejo University, Chiang Mai 50290, Thailand., Jinakote M; School of Human Kinetics and Health, Faculty of Health Science Technology, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal Academy, Bangkok 10210, Thailand., Srimaroeng C; Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. |
| Abstrakt: |
Coffea arabica pulp (CP) is a by-product of coffee processing. CP contains polyphenols that have exhibited beneficial effects, including antioxidant and lipid-lowering effects, as well as enhanced insulin sensitivity, in in vitro and in vivo models. How polyphenols, as found in CP aqueous extract (CPE), affect type 2 diabetes (T2D) has not been investigated. Thus, the present study examined the potential antidiabetic, antioxidant, and renoprotective effects of CPE-rich polyphenols, using an experimental model of T2D in rats induced by a high-fat diet and a single low dose of streptozotocin. The T2D rats received either 1000 mg/kg body weight (BW) of CPE, 30 mg/kg BW of metformin (Met), or a combination treatment (CPE + Met) for 3 months. Plasma parameters, kidney morphology and function, and renal organic transport were determined. Significant hyperglycemia, hypertriglyceridemia, insulin resistance, increased renal lipid content and lipid peroxidation, and morphological kidney changes related to T2D were restored by both CPE and CPE + Met treatments. Additionally, the renal uptake of organic cation, 3 H-1-methyl-4-phenylpyridinium (MPP + ), was reduced in T2D, while transport was restored by CPE and CPE + Met, through an up-regulation of antioxidant genes and protein kinase Cα deactivation. Thus, CPE has antidiabetic and antioxidant effects that potentially ameliorate kidney function in T2D by preserving renal organic cation transport through an oxidative stress pathway. |
