Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?
Autor: | Bergamo MT; Department of Pediatric Dentistry, Orthodontics and Collective Health, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, São Paulo, 17012-901, Brazil., Vitor LLR; Sacred Heart University, Bauru, São Paulo, Brazil., Dionísio TJ; Department of Biology Science, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, 17012-901, Brazil., Marques NCT; Department of Pediatric Dentistry, School of Dentistry, José do Rosário Vellano University, Alfenas, Minas Gerais, Brazil., Oliveira RC; Department of Biology Science, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, 17012-901, Brazil., Ambrosio ECP; Department of Pediatric Dentistry, Orthodontics and Collective Health, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, São Paulo, 17012-901, Brazil., Sakai VT; Department of Clinics and Surgery, School of Dentistry, Federal University of Alfenas, Alfenas, Minas Gerais, 37130 000, Brazil., Santos CF; Department of Biology Science, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, 17012-901, Brazil., Lourenço Neto N; Department of Pediatric Dentistry, Orthodontics and Collective Health, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, São Paulo, 17012-901, Brazil., Machado MAAM; Department of Pediatric Dentistry, Orthodontics and Collective Health, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, São Paulo, 17012-901, Brazil., Oliveira TM; Department of Pediatric Dentistry, Orthodontics and Collective Health, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, São Paulo, 17012-901, Brazil. marchini@usp.br. |
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Jazyk: | angličtina |
Zdroj: | Lasers in medical science [Lasers Med Sci] 2021 Oct; Vol. 36 (8), pp. 1751-1758. Date of Electronic Publication: 2021 Apr 01. |
DOI: | 10.1007/s10103-021-03291-4 |
Abstrakt: | This study aimed to evaluate the effect of different photobiomodulation (PBM) radiant exposures on the viability, proliferation, and gene expression of pulp fibroblasts from human primary teeth (HPF) involved in the pulp tissue repair. HPF were irradiated with Laser InGaAlP (Twin Flex Evolution, MMOptics®) at 660-nm wavelength (red); single time, continuous mode, 0.04-cm 2 laser tip area, and 0.225-cm laser tip diameter, keeping the distance of 1 mm between the laser beam and the cell culture. The doses used were between 1.2 and 6.2 J/cm 2 and were evaluated at the 6 h, 12 h, and 24 h after PBM. MTT and crystal violet assays evaluated the cell viability and proliferation. RT-PCR verified VEGF and FGF-2 mRNA expression. A blinded examiner analyzed the data through two-way ANOVA followed by Tukey test (p < 0.05). The groups with higher powers (10 mW, 15 mW, 20 mW, and 25 mW), shortest application periods (10 s), and radiant exposures between 2.5 and 6.2 J/cm 2 exhibited statistically higher viability than that of the groups with small power (5 mW), longer application period (50 s), and radiant exposure of 6.2 J/cm 2 (p < 0.05). VEGF and FGF-2 mRNA expression were observed at the three evaluated periods (6 h, 12 h, and 24 h) and the highest expression was in the shortest period (p < 0.05). All radiant exposures maintained HPF viable. The period of 6 h after irradiation showed statistically greater gene expression for both growth factors than other periods. VEGF mRNA had no differences among the dosimetries studied. The best radiant exposures for FGF-2 gene expression were 2.5 J/cm 2 and 3.7 J/cm 2 . (© 2021. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.) |
Databáze: | MEDLINE |
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