Integrating adaptive optics-SLO and OCT for multimodal visualization of the human retinal pigment epithelial mosaic.

Autor:	Bower AJ; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Liu T; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Aguilera N; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Li J; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Liu J; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Lu R; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Giannini JP; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Huryn LA; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA., Dubra A; Department of Ophthalmology, Stanford University, Palo Alto, CA 94303, USA., Liu Z; Center for Devices and Radiological Health (CDRH), U.S. Food and Drug Administration, 10903 New Hampshire Ave, Silver Spring, MD 20993, USA., Hammer DX; Center for Devices and Radiological Health (CDRH), U.S. Food and Drug Administration, 10903 New Hampshire Ave, Silver Spring, MD 20993, USA., Tam J; National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2021 Feb 17; Vol. 12 (3), pp. 1449-1466. Date of Electronic Publication: 2021 Feb 17 (Print Publication: 2021).
DOI:	10.1364/BOE.413438
Abstrakt:	In vivo imaging of human retinal pigment epithelial (RPE) cells has been demonstrated through multiple adaptive optics (AO)-based modalities. However, whether consistent and complete information regarding the cellular structure of the RPE mosaic is obtained across these modalities remains uncertain due to limited comparisons performed in the same eye. Here, an imaging platform combining multimodal AO-scanning light ophthalmoscopy (AO-SLO) with AO-optical coherence tomography (AO-OCT) is developed to make a side-by-side comparison of the same RPE cells imaged across four modalities: AO-darkfield, AO-enhanced indocyanine green (AO-ICG), AO-infrared autofluorescence (AO-IRAF), and AO-OCT. Co-registered images were acquired in five subjects, including one patient with choroideremia. Multimodal imaging provided multiple perspectives of the RPE mosaic that were used to explore variations in RPE cell contrast in a subject-, location-, and even cell-dependent manner. Estimated cell-to-cell spacing and density were found to be consistent both across modalities and with normative data. Multimodal images from a patient with choroideremia illustrate the benefit of using multiple modalities to infer the cellular structure of the RPE mosaic in an affected eye, in which disruptions to the RPE mosaic may locally alter the signal strength, visibility of individual RPE cells, or even source of contrast in unpredictable ways. Competing Interests: The authors declare that there are no conflicts of interest related to this article. Disclaimer: The mention of commercial products, their sources, or their use in connection with material reported herein is not to be construed as either an actual or implied endorsement of such products by the US Department of Health and Human Services. (© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.)
Databáze:	MEDLINE
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