Strain, Soil-Type, Irrigation Regimen, and Poultry Litter Influence Salmonella Survival and Die-off in Agricultural Soils.

Autor:	Bardsley CA; Department of Food Science and Technology, Eastern Shore Agricultural Research and Extension Center, Virginia Tech, Painter, VA, United States., Weller DL; Department of Environmental and Forest Biology, SUNY College of Environmental Science and Forestry, Syracuse, NY, United States., Ingram DT; Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD, United States., Chen Y; Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD, United States., Oryang D; Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD, United States., Rideout SL; School of Plant and Environmental Sciences, Eastern Shore Agricultural Research and Extension Center, Virginia Tech, Painter, VA, United States., Strawn LK; Department of Food Science and Technology, Eastern Shore Agricultural Research and Extension Center, Virginia Tech, Painter, VA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2021 Mar 16; Vol. 12, pp. 590303. Date of Electronic Publication: 2021 Mar 16 (Print Publication: 2021).
DOI:	10.3389/fmicb.2021.590303
Abstrakt:	The use of untreated biological soil amendments of animal origin (BSAAO) have been identified as one potential mechanism for the dissemination and persistence of Salmonella in the produce growing environment. Data on factors influencing Salmonella concentration in amended soils are therefore needed. The objectives here were to (i) compare die-off between 12 Salmonella strains following inoculation in amended soil and (ii) characterize any significant effects associated with soil-type, irrigation regimen, and amendment on Salmonella survival and die-off. Three greenhouse trials were performed using a randomized complete block design. Each strain (~4 log CFU/g) was homogenized with amended or non-amended sandy-loam or clay-loam soil. Salmonella levels were enumerated in 25 g samples 0, 0.167 (4 h), 1, 2, 4, 7, 10, 14, 21, 28, 56, 84, 112, 168, 210, 252, and 336 days post-inoculation (dpi), or until two consecutive samples were enrichment negative. Regression analysis was performed between strain, soil-type, irrigation, and (i) time to last detect (survival) and (ii) concentration at each time-point (die-off rate). Similar effects of strain, irrigation, soil-type, and amendment were identified using the survival and die-off models. Strain explained up to 18% of the variance in survival, and up to 19% of variance in die-off rate. On average Salmonella survived for 129 days in amended soils, however, Salmonella survived, on average, 30 days longer in clay-loam soils than sandy-loam soils [95% Confidence interval (CI) = 45, 15], with survival time ranging from 84 to 210 days for the individual strains during daily irrigation. When strain-specific associations were investigated using regression trees, S. Javiana and S. Saintpaul were found to survive longer in sandy-loam soil, whereas most of the other strains survived longer in clay-loam soil. Salmonella also survived, on average, 128 days longer when irrigated weekly, compared to daily (CI = 101, 154), and 89 days longer in amended soils, than non-amended soils (CI = 61, 116). Overall, this study provides insight into Salmonella survival following contamination of field soils by BSAAO. Specifically, Salmonella survival may be strain-specific as affected by both soil characteristics and management practices. These data can assist in risk assessment and strain selection for use in challenge and validation studies. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Bardsley, Weller, Ingram, Chen, Oryang, Rideout and Strawn.)
Databáze:	MEDLINE
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