Extracellular Vesicles From Epicardial Fat Facilitate Atrial Fibrillation.

Autor:	Shaihov-Teper O; Neufeld and Tamman Cardiovascular Research Institutes (O.S.-T., R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., J.L.), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Ram E; Department of Cardiac Surgery, Leviev Cardiothoracic and Vascular Center (E. Ram, E.M.Z., S.A., L.S., E. Raanani), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Ballan N; The Sohnis Family Laboratory for Cardiac Electrophysiology and Regenerative Medicine, Rappaport Faculty of Medicine, Technion Institute of Technology, Israel (N.B., L.G.)., Brzezinski RY; Neufeld and Tamman Cardiovascular Research Institutes (O.S.-T., R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., J.L.), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Naftali-Shani N; Neufeld and Tamman Cardiovascular Research Institutes (O.S.-T., R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., J.L.), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Masoud R; Cancer Research Center, Chaim Sheba Medical Center, Tel Hashomer, Israel (R.M.)., Ziv T; Smoler Proteomics Center, Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel (T.Z.)., Lewis N; Neufeld and Tamman Cardiovascular Research Institutes (O.S.-T., R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., J.L.), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Schary Y; Neufeld and Tamman Cardiovascular Research Institutes (O.S.-T., R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., J.L.), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Levin-Kotler LP; Neufeld and Tamman Cardiovascular Research Institutes (O.S.-T., R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., J.L.), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Volvovitch D; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Zuroff EM; Department of Cardiac Surgery, Leviev Cardiothoracic and Vascular Center (E. Ram, E.M.Z., S.A., L.S., E. Raanani), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Amunts S; Department of Cardiac Surgery, Leviev Cardiothoracic and Vascular Center (E. Ram, E.M.Z., S.A., L.S., E. Raanani), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Regev-Rudzki N; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel (N.R.-R.)., Sternik L; Department of Cardiac Surgery, Leviev Cardiothoracic and Vascular Center (E. Ram, E.M.Z., S.A., L.S., E. Raanani), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Raanani E; Department of Cardiac Surgery, Leviev Cardiothoracic and Vascular Center (E. Ram, E.M.Z., S.A., L.S., E. Raanani), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.)., Gepstein L; The Sohnis Family Laboratory for Cardiac Electrophysiology and Regenerative Medicine, Rappaport Faculty of Medicine, Technion Institute of Technology, Israel (N.B., L.G.)., Leor J; Neufeld and Tamman Cardiovascular Research Institutes (O.S.-T., R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., J.L.), Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.; Heart Center, Sheba Medical Center, Tel Hashomer, Israel (O.S.-T., E. Ram, R.Y.B., N.N.-S., N.L., Y.S., L.-P.L.-K., D.V., E.M.Z., S.A., L.S., E. Raanani, J.L.).
Jazyk:	angličtina
Zdroj:	Circulation [Circulation] 2021 Jun 22; Vol. 143 (25), pp. 2475-2493. Date of Electronic Publication: 2021 Apr 01.
DOI:	10.1161/CIRCULATIONAHA.120.052009
Abstrakt:	Background: The role of epicardial fat (eFat)-derived extracellular vesicles (EVs) in the pathogenesis of atrial fibrillation (AF) has never been studied. We tested the hypothesis that eFat-EVs transmit proinflammatory, profibrotic, and proarrhythmic molecules that induce atrial myopathy and fibrillation. Methods: We collected eFat specimens from patients with (n=32) and without AF (n=30) during elective heart surgery. eFat samples were grown as organ cultures, and the culture medium was collected every 2 days. We then isolated and purified eFat-EVs from the culture medium, and analyzed the EV number, size, morphology, specific markers, encapsulated cytokines, proteome, and microRNAs. Next, we evaluated the biological effects of unpurified and purified EVs on atrial mesenchymal stromal cells and endothelial cells in vitro. To establish a causal association between eFat-EVs and vulnerability to AF, we modeled AF in vitro using induced pluripotent stem cell-derived cardiomyocytes. Results: Microscopic examination revealed excessive inflammation, fibrosis, and apoptosis in fresh and cultured eFat tissues. Cultured explants from patients with AF secreted more EVs and harbored greater amounts of proinflammatory and profibrotic cytokines, and profibrotic microRNA, as well, than those without AF. The proteomic analysis confirmed the distinctive profile of purified eFat-EVs from patients with AF. In vitro, purified and unpurified eFat-EVs from patients with AF had a greater effect on proliferation and migration of human mesenchymal stromal cells and endothelial cells, compared with eFat-EVs from patients without AF. Last, whereas eFat-EVs from patients with and without AF shortened the action potential duration of induced pluripotent stem cell-derived cardiomyocytes, only eFat-EVs from patients with AF induced sustained reentry (rotor) in induced pluripotent stem cell-derived cardiomyocytes. Conclusions: We show, for the first time, a distinctive proinflammatory, profibrotic, and proarrhythmic signature of eFat-EVs from patients with AF. Our findings uncover another pathway by which eFat promotes the development of atrial myopathy and fibrillation.
Databáze:	MEDLINE
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