| Autor: |
Sanchez A; Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France., Maenner S; Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France. sylvain.maenner@univ-lorraine.fr. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2300, pp. 99-106. |
| DOI: |
10.1007/978-1-0716-1386-3_10 |
| Abstrakt: |
RiboNucleoProtein particles (RNPs), which are composed of RNAs and proteins, play essential roles in many biological processes. The isolation of these molecular machines is a critical step to better understand their mechanisms of action. In this chapter, we describe the MS2-MBP affinity chromatography used to purify the protein content of the RNPs formed with an RNA of interest in a nuclear extract. Substrate RNAs are furnished with a tag consisting of three stem-loops that provide specific binding sites for the phage MS2 protein. Here, we successfully applied this method to isolate RNPs formed with subfragments of the long noncoding RNA ANRIL (Antisense Noncoding RNA in the INK4 Locus). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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