Emergence of porcine delta-coronavirus pathogenic infections among children in Haiti through independent zoonoses and convergent evolution.

Autor: Lednicky JA; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL., Tagliamonte MS; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL., White SK; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL., Elbadry MA; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL., Alam MM; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL., Stephenson CJ; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL., Bonny TS; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL., Loeb JC; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL., Telisma T; Christianville Foundation, Gressier, Haiti., Chavannes S; Christianville Foundation, Gressier, Haiti., Ostrov DA; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL., Mavian C; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL., Beau De Rochars VM; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Health Services Research, Management and Policy, College of Public Health and Health Professions, University of Florida, Gainesville, FL., Salemi M; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL., Morris JG Jr; Emerging Pathogens Institute, University of Florida, Gainesville, FL.; Department of Medicine, College of Medicine, University of Florida, Gainesville, FL.
Jazyk: angličtina
Zdroj: MedRxiv : the preprint server for health sciences [medRxiv] 2021 Mar 25. Date of Electronic Publication: 2021 Mar 25.
DOI: 10.1101/2021.03.19.21253391
Abstrakt: Coronaviruses have caused three major epidemics since 2003, including the ongoing SARS-CoV-2 pandemic. In each case, coronavirus emergence in our species has been associated with zoonotic transmissions from animal reservoirs 1,2 , underscoring how prone such pathogens are to spill over and adapt to new species. Among the four recognized genera of the family Coronaviridae - Alphacoronavirus, Betacoronavirus, Deltacoronavirus, Gammacoronavirus , - human infections reported to date have been limited to alpha- and betacoronaviruses 3 . We identify, for the first time, porcine deltacoronavirus (PDCoV) strains in plasma samples of three Haitian children with acute undifferentiated febrile illness. Genomic and evolutionary analyses reveal that human infections were the result of at least two independent zoonoses of distinct viral lineages that acquired the same mutational signature in the nsp15 and the spike glycoprotein genes by convergent evolution. In particular, structural analysis predicts that one of the changes in the Spike S1 subunit, which contains the receptor-binding domain, may affect protein's flexibility and binding to the host cell receptor. Our findings not only underscore the ability of deltacoronaviruses to adapt and potentially lead to human-to-human transmission, but also raise questions about the role of such transmissions in development of pre-existing immunity to other coronaviruses, such as SARS-CoV-2.
Databáze: MEDLINE