In vitro and in vivo effects of P-MAPA immunomodulator on schistosomiasis.

Autor: Silva JCS; Faculty of Medical Sciences - University of Pernambuco (FCM/UPE). Arnóbio Marques street, 310, 50100-130, Recife, PE, Brazil., Lins CRB; Aggeu Magalhães Institute - Oswaldo Cruz Foundation (IAM/FIOCRUZ). Prof. Moraes Rego avenue, s/n, 50670-420, Recife, PE, Brazil., Lacerda SS; Aggeu Magalhães Institute - Oswaldo Cruz Foundation (IAM/FIOCRUZ). Prof. Moraes Rego avenue, s/n, 50670-420, Recife, PE, Brazil., Ramos REM; Aggeu Magalhães Institute - Oswaldo Cruz Foundation (IAM/FIOCRUZ). Prof. Moraes Rego avenue, s/n, 50670-420, Recife, PE, Brazil., Araújo HDA; Department of Biochemistry, Biosciences Centre - Federal University of Pernambuco (UFPE). Prof. Moraes Rego avenue, 1235, 50670-420, Recife, PE, Brazil., Melo-Junior MR; Health Sciences Centre - Federal University of Pernambuco (UFPE). Prof. Moraes Rego avenue, 1235, 50670-501, Recife, PE, Brazil., Alves LC; Aggeu Magalhães Institute - Oswaldo Cruz Foundation (IAM/FIOCRUZ). Prof. Moraes Rego avenue, s/n, 50670-420, Recife, PE, Brazil., Brayner FA; Aggeu Magalhães Institute - Oswaldo Cruz Foundation (IAM/FIOCRUZ). Prof. Moraes Rego avenue, s/n, 50670-420, Recife, PE, Brazil., Nunes IS; Farmabrasilis R&D Division. Av. Hélio Pires de Camargo 181, 13279-020, Valinhos, SP, Brazil., Melo FL; Aggeu Magalhães Institute - Oswaldo Cruz Foundation (IAM/FIOCRUZ). Prof. Moraes Rego avenue, s/n, 50670-420, Recife, PE, Brazil., Carvalho BM; Faculty of Medical Sciences - University of Pernambuco (FCM/UPE). Arnóbio Marques street, 310, 50100-130, Recife, PE, Brazil; Institute of Biological Sciences - University of Pernambuco (ICB/UPE). Arnóbio Marques street, 310, 50100-130, Recife, PE, Brazil. Electronic address: bruno.carvalho@upe.br.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2021 Jun; Vol. 218, pp. 105909. Date of Electronic Publication: 2021 Mar 28.
DOI: 10.1016/j.actatropica.2021.105909
Abstrakt: Schistosomiasis is an infectious disease caused by helminth parasites of the genus Schistosoma; it is transmitted in over 78 countries. The main strategy for schistosomiasis control is treatment of infected people with praziquantel (PZQ). As PZQ-resistant strains have emerged, new anti-schistosomal agents have become necessary. We evaluated the in vitro and in vivo effect of P-MAPA, an aggregated polymer of protein magnesium ammonium phospholinoleate-palmitoleate anhydride with immunomodulatory properties; it is produced by Aspergillus oryzae fermentation. In vitro, P-MAPA (5, 50, and 100 µg/mL) damaged the Schistosoma mansoni tegument, causing thorn losses and tuber destruction in male worms and peeling and erosion in females after 24-h incubation. In vivo, P-MAPA (5 and 100 mg/kg, alone and combined with PZQ - 50 mg/kg) reduced the number of eggs by up to 69.20% in the liver and 88.08% in the intestine. Furthermore, granulomas were reduced up to 83.13%, and there was an increase in the number of dead eggs and a reduction of serum aspartate aminotransferase levels. These data suggest that P-MAPA activity can help improve schistosomiasis treatment and patients' quality of life.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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