Altered chromatin conformation and transcriptional regulation in watermelon following genome doubling.

Autor: Garcia-Lozano M; Department of Biology, Gus R. Douglass Institute, West Virginia State University Institute, Charleston, WV, USA., Natarajan P; Department of Biology, Gus R. Douglass Institute, West Virginia State University Institute, Charleston, WV, USA., Levi A; USDA, ARS, U.S. Vegetable Lab, Charleston, SC, USA., Katam R; Department of Biological Sciences, Florida A&M University, Tallahassee, FL, USA., Lopez-Ortiz C; Department of Biology, Gus R. Douglass Institute, West Virginia State University Institute, Charleston, WV, USA., Nimmakayala P; Department of Biology, Gus R. Douglass Institute, West Virginia State University Institute, Charleston, WV, USA., Reddy UK; Department of Biology, Gus R. Douglass Institute, West Virginia State University Institute, Charleston, WV, USA.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2021 May; Vol. 106 (3), pp. 588-600. Date of Electronic Publication: 2021 Apr 22.
DOI: 10.1111/tpj.15256
Abstrakt: Polyploidy has played a crucial role in plant evolution, development and function. Synthetic autopolyploid represents an ideal system to investigate the effects of polyploidization on transcriptional regulation. In this study, we deciphered the impact of genome duplication at phenotypic and molecular levels in watermelon. Overall, 88% of the genes in tetraploid watermelon followed a >1:1 dosage effect, and accordingly, differentially expressed genes were largely upregulated. In addition, a great number of hypomethylated regions (1688) were identified in an isogenic tetraploid watermelon. These differentially methylated regions were localized in promoters and intergenic regions and near transcriptional start sites of the identified upregulated genes, which enhances the importance of methylation in gene regulation. These changes were reflected in sophisticated higher-order chromatin structures. The genome doubling caused switching of 108 A and 626 B compartments that harbored genes associated with growth, development and stress responses.
(© 2021 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: