Phytochemical characterization and inhibition of Candida sp. by the essential oil of Baccharis trimera (Less.) DC.

Autor: da Silva TG; Department of Biological Chemistry, Universidade Regional do Cariri, URCA, Av. Cel. Antonio Luiz, 1161, 63105-000, Crato, CE, Brasil., da Silva JCP; Department of Biological Chemistry, Universidade Regional do Cariri, URCA, Av. Cel. Antonio Luiz, 1161, 63105-000, Crato, CE, Brasil., Carneiro JNP; Department of Biological Chemistry, Universidade Regional do Cariri, URCA, Av. Cel. Antonio Luiz, 1161, 63105-000, Crato, CE, Brasil., do Amaral W; Universidade Federal Do Paraná, UFPR, Curitiba, PR, Brasil., Deschamps C; Universidade Federal Do Paraná, UFPR, Curitiba, PR, Brasil., de Araújo JP; Universidade Federal Do Paraná, UFPR, Curitiba, PR, Brasil., da Costa JGM; Department of Biological Chemistry, Universidade Regional do Cariri, URCA, Av. Cel. Antonio Luiz, 1161, 63105-000, Crato, CE, Brasil., de Oliveira Almeida W; Department of Biological Chemistry, Universidade Regional do Cariri, URCA, Av. Cel. Antonio Luiz, 1161, 63105-000, Crato, CE, Brasil., da Silva LE; Universidade Federal Do Paraná, UFPR, Curitiba, PR, Brasil., Coutinho HDM; Department of Biological Chemistry, Universidade Regional do Cariri, URCA, Av. Cel. Antonio Luiz, 1161, 63105-000, Crato, CE, Brasil. hdmcoutinho@gmail.com., Filho JR; Fundação Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, BA, Brasil., Morais-Braga MFB; Department of Biological Chemistry, Universidade Regional do Cariri, URCA, Av. Cel. Antonio Luiz, 1161, 63105-000, Crato, CE, Brasil.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2021 Aug; Vol. 203 (6), pp. 3077-3087. Date of Electronic Publication: 2021 Mar 31.
DOI: 10.1007/s00203-021-02304-8
Abstrakt: This study aimed to investigate the chemical composition and antifungal potential of the essential oil of Baccharis trimera (Less.) DC. against Candida strains. The half maximal inhibitory concentration (IC 50 ) was assessed by the microdilution method using the essential oil at a concentration range of 8192 to 8 μg/mL. The minimum fungicide concentration (MFC) was determined by subculture in solid medium. The ability of the essential oil to modulate the activity of antifungals was determined in wells treated simultaneously with the oil at a subinhibitory concentration (MFC/16) and fluconazole (FCZ). The fungal morphology was analyzed by microscopy. Gas chromatography coupled with mass spectrometry (GC/MS) was used to identify the chemical composition. The essential oil presented an CI 50 of 11.24 and 1.45 μg/mL, which was found to potentiate the effect of FCZ against Candida albicans. On the other hand, this combined treatment resulted in antagonism against Candida tropicalis and no evident modulation against Candida krusei was observed. The essential oil significantly inhibited hyphae growth. However, with a MFC ≥ 16,384 μg/mL, it is assumed that it has a fungistatic action. The antifungal properties demonstrated in this study might be related to the presence of sesquiterpenes and monoterpenes, and the interaction between them. In conclusion, Baccharis trimera showed promising anti-Candida effects, in addition to potentiating the activity of FCZ against Candida albicans, affecting its morphological transition. Therefore, this species constitutes a source of chemical compounds with the potential to be used in the combat of fungal infections.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: