Modulation of the innate immune response, antioxidant system and oxidative stress during acute and chronic stress in pacu (Piaractus mesopotamicus).

Autor: de Fátima Pereira de Faria C; Universidade Estadual Paulista, UNESP, Centro de Aquicultura, Via de Acesso Paulo Donato Castellane, 14.884-900, Jaboticabal, SP, Brazil., Dos Reis Martinez CB; Universidade Estadual de Londrina, UEL, Departamento de Ciências Fisiológicas, Rodovia Celso Garcia Cid - Pr 445 Km 380, Campus Universitário, Londrina, PR, 86057-970, Brazil., Takahashi LS; Universidade Estadual Paulista, UNESP, Centro de Aquicultura, Via de Acesso Paulo Donato Castellane, 14.884-900, Jaboticabal, SP, Brazil.; Universidade Estadual Paulista, UNESP, Faculdade de Ciências Agrárias e Tecnologia (FCAT-Unesp), Dracena, Rod. João Ribeiro de Barros, km 651, Dracena, SP, 17900-000, Brazil., de Mello MMM; Universidade Estadual Paulista, UNESP, Centro de Aquicultura, Via de Acesso Paulo Donato Castellane, 14.884-900, Jaboticabal, SP, Brazil., Martins TP; Universidade Estadual Paulista, UNESP, Centro de Aquicultura, Via de Acesso Paulo Donato Castellane, 14.884-900, Jaboticabal, SP, Brazil., Urbinati EC; Universidade Estadual Paulista, UNESP, Centro de Aquicultura, Via de Acesso Paulo Donato Castellane, 14.884-900, Jaboticabal, SP, Brazil. elisabeth.criscuolo-urbinati@unesp.br.; Universidade Estadual Paulista, UNESP, Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Paulo Donato Castellane, 14, Jaboticabal, SP, .884-900, Brazil. elisabeth.criscuolo-urbinati@unesp.br.
Jazyk: angličtina
Zdroj: Fish physiology and biochemistry [Fish Physiol Biochem] 2021 Aug; Vol. 47 (4), pp. 895-905. Date of Electronic Publication: 2021 Mar 31.
DOI: 10.1007/s10695-021-00940-x
Abstrakt: Stress is an energy-demanding process, as well as the responses of the innate immune system, that impose a metabolic overload on cellular energy production, which can affect the cellular redox balance, causing oxidative damage. We evaluated the role of stress in the modulation of innate immune and oxidative/antioxidant mechanisms in juvenile pacu exposed to acute and chronic stressors. The experimental period lasted 30 days, and fish (113.7 ± 35.1 g) were fed commercial feed. During this period, half of the fish were not manipulated (Condition A), and the other half were chased with a dip net for 5 min twice a day (Condition C). After the 30-day period, fish from both groups were sampled (baseline sampling), and the remainders (not sampled) were air exposed for 3 min (acute stressor), returned to the tanks, and were sampled again 30 min, 3 h, 6 h, and 24 h after air exposure. We evaluated biomarkers of stress (circulating cortisol and glucose), the innate immune system (respiratory burst activity/RBA, hemolytic activity of the complement system (HA-AP) and serum concentration of lysozyme), oxidative damage (lipid peroxidation/LPO), and antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; and glutathione peroxidase, GSH-Px). Our results showed that stress, acutely or chronically, caused a transient reduction of RAL and activated the HA-AP. Acutely, stress increased the lysozyme concentration. Furthermore, both conditions caused oxidative stress in the liver, and differently they modulated the antioxidant system, enhancing SOD activity and impairing CAT and GSH-Px activity.
(© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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