Microsatellite-Based Genotyping, Analysis of Population Structure, Presence of Trichomonas vaginalis Virus (TVV) and Mycoplasma hominis in T. vaginalis Isolates from Southwest of Turkey.

Autor:	Ertabaklar H; Department of Parasitology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey., Malatyali E; Department of Parasitology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey., Özün Özbay EP; Department of Obstetrics and Gynecology, Ege-Liva Hospital, Aydin, Turkey., Yildiz İ; Department of Parasitology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey., Sinecen M; Department of Computer Engineering, Faculty of Engineering, Adnan Menderes University, Aydin, Turkey., Ertuğ S; Department of Parasitology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey., Bozdoğan B; Department of Medical Microbiology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.; Recombinant DNA and Recombinant Protein Research Center (REDPROM), Adnan Menderes University, Aydin, Turkey., Güçlü Ö; Recombinant DNA and Recombinant Protein Research Center (REDPROM), Adnan Menderes University, Aydin, Turkey.; Department of Plant and Animal Production, Sultanhisar MYO, Adnan Menderes University, Aydin, Turkey.
Jazyk:	angličtina
Zdroj:	Iranian journal of parasitology [Iran J Parasitol] 2021 Jan-Mar; Vol. 16 (1), pp. 81-90.
DOI:	10.18502/ijpa.v16i1.5515
Abstrakt:	Background: The present study aimed to determine genetic diversity of Trichomonas vaginalis (T. vaginalis) isolates with microsatellite markers in Turkey (Nov 2015 to 2016) and to create a web-based microsatellite typing (MT) approach for the global interpretation of the data. In addition, the endosymbiosis of Mycoplasma hominis (M. hominis) and T. vaginalis virus (TVV) in the isolates was also examined. Methods: The allele sizes for each locus were calculated and microsatellite types were determined according to the allele profiles. The population structure was examined with Bayesian clustering method. A website (http://mttype.adu.edu.tr) was created for collection and sharing of microsatellite data. Presence of TVV and M. hominis in T. vaginalis isolates were investigated with electrophoresis and PCR. Results: Of 630 vaginal samples T. vaginalis was detected in 30 (4.7%) and those were used for further analysis. The structure produced by a clustering algorithm revealed eight genetic groups. The typing of isolates according to microsatellites revealed 23 different microsatellite types. Three clones were determined among isolates (MT10 16.7%; MT18 10% and MT3 6.7%). The frequency of TVV and M. hominis was 16.6% (n=5) and 20% (n=6), respectively. Conclusion: Presence of three clones among 30 T. vaginalis isolates indicated that microsatellite-based genotyping was efficient to determine the clonal distribution of T. vaginalis isolates. Therefore, a promising tool might be developed further and adapted to the studies dealing with molecular epidemiology of T. vaginalis . Microsatellite data from forthcoming studies will be deposited and presented on the website. In addition, we also presented the frequency of two endosymbionts in T. vaginalis isolates for the first time in Turkey. Competing Interests: Conflict of interest The authors declare that there is no conflict of interest. (Copyright © 2021 Ertabaklar et al. Published by Tehran University of Medical Sciences.)
Databáze:	MEDLINE
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