CRISPR based development of RNA editing and the diagnostic platform.

Autor:	Satish L; Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel; The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Bergman Campus, Beer Sheva, Israel., Lavanya G; Department of Postharvest and Fresh Produce, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel., Kasthuri T; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India., Kalaivaani A; Department of Postharvest and Fresh Produce, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel., Shamili S; The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Bergman Campus, Beer Sheva, Israel., Muthuramalingam P; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India., Gowrishankar S; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India., Pandian SK; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India., Singh V; Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, Gujarat, India., Sitrit Y; The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Bergman Campus, Beer Sheva, Israel., Kushmaro A; Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel. Electronic address: arielkus@bgu.ac.il.
Jazyk:	angličtina
Zdroj:	Progress in molecular biology and translational science [Prog Mol Biol Transl Sci] 2021; Vol. 179, pp. 117-159. Date of Electronic Publication: 2021 Feb 15.
DOI:	10.1016/bs.pmbts.2020.12.015
Abstrakt:	Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system has improved the ability to edit and control gene expression as desired. Genome editing approaches are currently leading the biomedical research with improved focus on direct nuclease dependent editing. So far, the research was predominantly intended on genome editing over the DNA level, recent adapted techniques are initiating to secure momentum through their proficiency to provoke modifications in RNA sequence. Integration of this system besides to lateral flow method allows reliable, quick, sensitive, precise and inexpensive diagnostic. These interesting methods illustrate only a small proportion of what is technically possible for this novel technology, but several technological obstacles need to be overcome prior to the CRISPR-Cas genome editing system can meet its full ability. This chapter covers the particulars on recent advances in CRISPR-Cas9 genome editing technology including diagnosis and technical advancements, followed by molecular mechanism of CRISPR-based RNA editing and diagnostic tools and types, and CRISPR-Cas-based biosensors. (Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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