Genetic requirements for cell division in a genomically minimal cell.

Autor:	Pelletier JF; Center for Bits and Atoms, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; National Institute of Standards and Technology, Gaithersburg, MD 20899, USA., Sun L; J. Craig Venter Institute, La Jolla, CA 92037, USA., Wise KS; J. Craig Venter Institute, La Jolla, CA 92037, USA., Assad-Garcia N; J. Craig Venter Institute, La Jolla, CA 92037, USA., Karas BJ; Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 5C1, Canada., Deerinck TJ; National Center for Microscopy and Imaging Research, University of California-San Diego, La Jolla, CA 92037, USA., Ellisman MH; National Center for Microscopy and Imaging Research, University of California-San Diego, La Jolla, CA 92037, USA., Mershin A; Center for Bits and Atoms, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Gershenfeld N; Center for Bits and Atoms, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Chuang RY; J. Craig Venter Institute, La Jolla, CA 92037, USA., Glass JI; J. Craig Venter Institute, La Jolla, CA 92037, USA. Electronic address: jglass@jcvi.org., Strychalski EA; National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. Electronic address: elizabeth.strychalski@nist.gov.
Jazyk:	angličtina
Zdroj:	Cell [Cell] 2021 Apr 29; Vol. 184 (9), pp. 2430-2440.e16. Date of Electronic Publication: 2021 Mar 29.
DOI:	10.1016/j.cell.2021.03.008
Abstrakt:	Genomically minimal cells, such as JCVI-syn3.0, offer a platform to clarify genes underlying core physiological processes. Although this minimal cell includes genes essential for population growth, the physiology of its single cells remained uncharacterized. To investigate striking morphological variation in JCVI-syn3.0 cells, we present an approach to characterize cell propagation and determine genes affecting cell morphology. Microfluidic chemostats allowed observation of intrinsic cell dynamics that result in irregular morphologies. A genome with 19 genes not retained in JCVI-syn3.0 generated JCVI-syn3A, which presents morphology similar to that of JCVI-syn1.0. We further identified seven of these 19 genes, including two known cell division genes, ftsZ and sepF, a hydrolase of unknown substrate, and four genes that encode membrane-associated proteins of unknown function, which are required together to restore a phenotype similar to that of JCVI-syn1.0. This result emphasizes the polygenic nature of cell division and morphology in a genomically minimal cell. Competing Interests: Declaration of interests The authors declare no competing interests. (Published by Elsevier Inc.)
Databáze:	MEDLINE
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