A proctolin-like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion.

Autor: Llopis-Giménez A; Department of Genetics and Institut Universitari en Biotecnologia i Biomedicina (BIOTECMED), Universitat de València, Valencia, Spain., Parenti S; Department of Genetics and Institut Universitari en Biotecnologia i Biomedicina (BIOTECMED), Universitat de València, Valencia, Spain., Han Y; Laboratory of Virology, Wageningen University and Research, Wageningen, the Netherlands.; Current address. Department of Pathology, University of Cambridge, Cambridge, UK., Ros VID; Laboratory of Virology, Wageningen University and Research, Wageningen, the Netherlands., Herrero S; Department of Genetics and Institut Universitari en Biotecnologia i Biomedicina (BIOTECMED), Universitat de València, Valencia, Spain.
Jazyk: angličtina
Zdroj: Insect science [Insect Sci] 2022 Feb; Vol. 29 (1), pp. 230-244. Date of Electronic Publication: 2021 Mar 29.
DOI: 10.1111/1744-7917.12913
Abstrakt: Baculoviruses constitute a large group of invertebrate DNA viruses, predominantly infecting larvae of the insect order Lepidoptera. During a baculovirus infection, the virus spreads throughout the insect body producing a systemic infection in multiple larval tissues, included the central nervous system (CNS). As a main component of the CNS, neuropeptides are small protein-like molecules functioning as neurohormones, neurotransmitters, or neuromodulators. These peptides are involved in regulating animal physiology and behavior and could be altered after baculovirus infection. In this study, we have investigated the effect of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) infection on expression of Spodoptera exigua neuropeptides and neuropeptide-like genes. Expression of the gene encoding a polypeptide that resembles the well-known insect neuropeptide proctolin and named as proctolin-like peptide (PLP), was downregulated in the larval brain following infection and was chosen for further analysis. A recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) overexpressing the C-terminal part of the PLP was generated and used in bioassays using S. exigua larvae to study its influence on the viral infection and insect behavior. AcMNPV-PLP-infected larvae showed less locomotion activity and a reduction in growth compared to larvae infected with wild type AcMNPV or mock-infected larvae. These results are indicative of this new peptide as a neuromodulator that regulates visceral and skeletal muscle contractions and offers a novel effector involved in the behavioral changes during baculovirus infection.
(© 2021 Institute of Zoology, Chinese Academy of Sciences.)
Databáze: MEDLINE
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