Comparative analysis of biofilm models to determine the efficacy of antimicrobials.

Autor: Stuermer EK; Dept. of Vascular Medicine, University Heart Center, University Medical Center Hamburg-Eppendorf (UKE), Martini Street 52, 20246, Hamburg, Germany. Electronic address: e.stuermer@uke.de., Besser M; Dpt. of Translational Wound Research, Centre for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Street 10, Witten, Germany., Brill F; Dr. Brill + Partner GmbH, Institute for Hygiene and Microbiology, Stiegstueck 34, 22339, Hamburg, Germany., Geffken M; Institute for Transfusion Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Plattfaut I; Dpt. of Translational Wound Research, Centre for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Street 10, Witten, Germany., Severing AL; Dpt. of Translational Wound Research, Centre for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Street 10, Witten, Germany., Wiencke V; Dr. Brill + Partner GmbH, Institute for Hygiene and Microbiology, Stiegstueck 34, 22339, Hamburg, Germany., Rembe JD; Dpt. of Translational Wound Research, Centre for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Street 10, Witten, Germany; Dpt. of Vascular and Endovascular Surgery, Heinrich-Heine-University of Düsseldorf, Moorenstreet 5, 40225, Düsseldorf, Germany., Naumova EA; Department of Biological and Material Sciences in Dentistry, School of Dentistry, Faculty of Health, Witten/Herdecke University, Witten, Germany., Kampe A; Dr. Brill + Partner GmbH, Institute for Hygiene and Microbiology, Stiegstueck 34, 22339, Hamburg, Germany., Debus S; Dept. of Vascular Medicine, University Heart Center, University Medical Center Hamburg-Eppendorf (UKE), Martini Street 52, 20246, Hamburg, Germany., Smeets R; Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Martini Street 52, 20246, Hamburg, Germany.
Jazyk: angličtina
Zdroj: International journal of hygiene and environmental health [Int J Hyg Environ Health] 2021 May; Vol. 234, pp. 113744. Date of Electronic Publication: 2021 Mar 26.
DOI: 10.1016/j.ijheh.2021.113744
Abstrakt: Biofilms are one of the greatest challenges in today's treatment of chronic wounds. While antimicrobials kill platonic bacteria within seconds, they are rarely able to harm biofilms. In order to identify effective substances for antibacterial therapy, cost-efficient, standardized and reproducible models that aim to mimic the clinical situation are required. In this study, two 3D biofilm models based on human plasma with immune cells (lhBIOM) or based on sheep blood (sbBIOM) containing S. aureus or P. aeruginosa, are compared with the human biofilm model hpBIOM regarding their microscopic structure (scanning electron microscopy; SEM) and their bacterial resistance to octenidine hydrochloride (OCT) and a sodium hypochlorite (NaOCl) wound-irrigation solution. The three analyzed biofilm models show little to no reaction to treatment with the hypochlorous solution while planktonic S. aureus and P. aeruginosa cells are reduced within minutes. After 48 h, octenidine hydrochloride manages to erode the biofilm matrix and significantly reduce the bacterial load. The determined effects are qualitatively reflected by SEM. Our results show that both ethically acceptable human and sheep blood based biofilm models can be used as a standard for in vitro testing of new antimicrobial substances. Due to their composition, both fulfill the criteria of a reality-reflecting model and therefore should be used in the approval for new antimicrobial agents.
(Copyright © 2021 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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